Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries

B. A. Ball; A. J. Conley; D. T. MacLaughlin; S. A. Grundy; K. Sabeur; I. K.M. Liu

doi:10.1016/j.theriogenology.2008.05.059

Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries

B. A. Ball, A. J. Conley, D. T. MacLaughlin, S. A. Grundy, K. Sabeur, I. K.M. Liu

Veterinary Science

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of Müllerian duct development in the fetal rat. Immunolabeling with α-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-Müllerian hormone may be a useful biomarker for detection of GCT in the horse.

Original language	English
Pages (from-to)	968-977
Number of pages	10
Journal	Theriogenology
Volume	70
Issue number	6
DOIs	https://doi.org/10.1016/j.theriogenology.2008.05.059
State	Published - Oct 1 2008

Bibliographical note

Funding Information:
Supported by the John P. Hughes Endowment and the Center for Equine Health, with funds provided by the State of California Pari-mutuel Fund, and contributions by private donors. The authors thank Ms. Andrea Brum for assistance with immunohistochemistry, Dr. Anja Stoll and Dr. Alejandro Esteller Vico for providing some of the tissues used in this study, as well as Ms. Coralie Munro for assistance with endocrine assays.

Keywords

Anti-Müllerian hormone
Equine
Granulosa-cell tumor
Ovarian follicle
Ovary

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

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10.1016/j.theriogenology.2008.05.059

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@article{bccaf6ef4bca468d91fd735369fa9819,

title = "Expression of anti-M{\"u}llerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries",

abstract = "Anti-M{\"u}llerian hormone (AMH), also known as M{\"u}llerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of M{\"u}llerian duct development in the fetal rat. Immunolabeling with α-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-M{\"u}llerian hormone may be a useful biomarker for detection of GCT in the horse.",

keywords = "Anti-M{\"u}llerian hormone, Equine, Granulosa-cell tumor, Ovarian follicle, Ovary",

author = "Ball, {B. A.} and Conley, {A. J.} and MacLaughlin, {D. T.} and Grundy, {S. A.} and K. Sabeur and Liu, {I. K.M.}",

note = "Funding Information: Supported by the John P. Hughes Endowment and the Center for Equine Health, with funds provided by the State of California Pari-mutuel Fund, and contributions by private donors. The authors thank Ms. Andrea Brum for assistance with immunohistochemistry, Dr. Anja Stoll and Dr. Alejandro Esteller Vico for providing some of the tissues used in this study, as well as Ms. Coralie Munro for assistance with endocrine assays. ",

year = "2008",

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doi = "10.1016/j.theriogenology.2008.05.059",

language = "English",

volume = "70",

pages = "968--977",

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T1 - Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries

AU - Ball, B. A.

AU - Conley, A. J.

AU - MacLaughlin, D. T.

AU - Grundy, S. A.

AU - Sabeur, K.

AU - Liu, I. K.M.

N1 - Funding Information: Supported by the John P. Hughes Endowment and the Center for Equine Health, with funds provided by the State of California Pari-mutuel Fund, and contributions by private donors. The authors thank Ms. Andrea Brum for assistance with immunohistochemistry, Dr. Anja Stoll and Dr. Alejandro Esteller Vico for providing some of the tissues used in this study, as well as Ms. Coralie Munro for assistance with endocrine assays.

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of Müllerian duct development in the fetal rat. Immunolabeling with α-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-Müllerian hormone may be a useful biomarker for detection of GCT in the horse.

AB - Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of Müllerian duct development in the fetal rat. Immunolabeling with α-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-Müllerian hormone may be a useful biomarker for detection of GCT in the horse.

KW - Anti-Müllerian hormone

KW - Equine

KW - Granulosa-cell tumor

KW - Ovarian follicle

KW - Ovary

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SN - 0093-691X

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JO - Theriogenology

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IS - 6

ER -

Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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