Expression of anti-Müllerian hormone (AMH) in the equine testis

B. A. Ball; A. J. Conley; S. A. Grundy; K. Sabeur; I. K.M. Liu

doi:10.1016/j.theriogenology.2007.11.009

Expression of anti-Müllerian hormone (AMH) in the equine testis

B. A. Ball, A. J. Conley, S. A. Grundy, K. Sabeur, I. K.M. Liu

Veterinary Science

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Anti-Müllerian hormone (AMH) induces regression of Müllerian ducts during male fetal development; in the human male, it is expressed in Sertoli cells during fetal development (and through puberty). The objective was to characterize expression of AMH in the fetal, neonatal, prepubertal, and adult equine testis, as well as in equine cryptorchid testes, in select testicular neoplasms, and in intersex gonads, based upon immunohistochemistry (IHC). Testes were removed from equine fetuses at 5.5, 10, and 11 months of gestation, at 12 months of age, and from adult stallions. In addition, cryptorchid testes, testis tumors (teratomas, seminomas, Sertoli cell tumors), and male intersex gonads were examined by IHC for expression of AMH using a goat polyclonal primary antibody (α-AMH) directed against a C-terminal peptide antigen from human AMH. Immunolabeling with α-AMH was localized to Sertoli cells within the developing seminiferous tubules of fetal, neonatal and prepubertal equine testes, with no expression detected in Sertoli cells from normal adult equine testes. Furthermore, expression was detected in cryptorchid testes (in animals up to 3-4 years of age) and in Sertoli cell tumors and male intersex gonads. In conclusion, AMH was strongly expressed by Sertoli cells in fetal, neonatal and prepubertal equine testes, but not in normal adult testes. That AMH was expressed in cryptorchid testes may provide a useful biomarker for detection of cryptorchid testes, as well as for immunohistochemical characterization of testicular tumors and intersex gonads in the horse.

Original language	English
Pages (from-to)	624-631
Number of pages	8
Journal	Theriogenology
Volume	69
Issue number	5
DOIs	https://doi.org/10.1016/j.theriogenology.2007.11.009
State	Published - Mar 15 2008

Funding

Supported by the John P. Hughes Endowment and the Center for Equine Health, with funds provided by the Oak Tree Racing Association, the State of California Pari-mutuel Fund, and contributions by private donors. The authors thank Dr. J. Brown and Dr. A. Hodder for providing the fetal tissues used in this study, as well as Ms. Andrea Brum for assistance with immunohistochemistry.

Funders	Funder number
John P. Hughes Endowment
Oak Tree Racing Association
State of California Pari-Mutuel Fund

Keywords

Anti-Müllerian hormone
Cryptorchidism
Equine
Sertoli cell
Testis

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

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10.1016/j.theriogenology.2007.11.009

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title = "Expression of anti-M{\"u}llerian hormone (AMH) in the equine testis",

abstract = "Anti-M{\"u}llerian hormone (AMH) induces regression of M{\"u}llerian ducts during male fetal development; in the human male, it is expressed in Sertoli cells during fetal development (and through puberty). The objective was to characterize expression of AMH in the fetal, neonatal, prepubertal, and adult equine testis, as well as in equine cryptorchid testes, in select testicular neoplasms, and in intersex gonads, based upon immunohistochemistry (IHC). Testes were removed from equine fetuses at 5.5, 10, and 11 months of gestation, at 12 months of age, and from adult stallions. In addition, cryptorchid testes, testis tumors (teratomas, seminomas, Sertoli cell tumors), and male intersex gonads were examined by IHC for expression of AMH using a goat polyclonal primary antibody (α-AMH) directed against a C-terminal peptide antigen from human AMH. Immunolabeling with α-AMH was localized to Sertoli cells within the developing seminiferous tubules of fetal, neonatal and prepubertal equine testes, with no expression detected in Sertoli cells from normal adult equine testes. Furthermore, expression was detected in cryptorchid testes (in animals up to 3-4 years of age) and in Sertoli cell tumors and male intersex gonads. In conclusion, AMH was strongly expressed by Sertoli cells in fetal, neonatal and prepubertal equine testes, but not in normal adult testes. That AMH was expressed in cryptorchid testes may provide a useful biomarker for detection of cryptorchid testes, as well as for immunohistochemical characterization of testicular tumors and intersex gonads in the horse.",

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AU - Sabeur, K.

AU - Liu, I. K.M.

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Expression of anti-Müllerian hormone (AMH) in the equine testis

Abstract

Funding

Keywords

ASJC Scopus subject areas

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