Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli

Robert J. Danaher, Daniel C. Stein

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalGene
Volume89
Issue number1
DOIs
StatePublished - Apr 30 1990

Bibliographical note

Funding Information:
This work was funded by a grant from the U.S. Navy to the University of Maryland through the University Research Initiative Program, grant No. N 00014-86K-0696.

Keywords

  • EcoRV isoschizomers
  • Recombinant DNA
  • enzymes
  • hydrothermal vents methyltransferases
  • marine bacterium

ASJC Scopus subject areas

  • Genetics

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