A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.
|Number of pages||4|
|State||Published - Apr 30 1990|
Bibliographical noteFunding Information:
This work was funded by a grant from the U.S. Navy to the University of Maryland through the University Research Initiative Program, grant No. N 00014-86K-0696.
- EcoRV isoschizomers
- Recombinant DNA
- hydrothermal vents methyltransferases
- marine bacterium
ASJC Scopus subject areas