Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli

Robert J. Danaher; Daniel C. Stein

doi:10.1016/0378-1119(90)90215-D

Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli

Robert J. Danaher, Daniel C. Stein

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.

Original language	English
Pages (from-to)	129-132
Number of pages	4
Journal	Gene
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(90)90215-D
State	Published - Apr 30 1990

Bibliographical note

Funding Information:
This work was funded by a grant from the U.S. Navy to the University of Maryland through the University Research Initiative Program, grant No. N 00014-86K-0696.

Keywords

EcoRV isoschizomers
Recombinant DNA
enzymes
hydrothermal vents methyltransferases
marine bacterium

ASJC Scopus subject areas

Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0378-1119(90)90215-D

Cite this

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title = "Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli",

abstract = "A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.",

keywords = "EcoRV isoschizomers, Recombinant DNA, enzymes, hydrothermal vents methyltransferases, marine bacterium",

author = "Danaher, {Robert J.} and Stein, {Daniel C.}",

note = "Funding Information: This work was funded by a grant from the U.S. Navy to the University of Maryland through the University Research Initiative Program, grant No. N 00014-86K-0696.",

year = "1990",

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doi = "10.1016/0378-1119(90)90215-D",

language = "English",

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T1 - Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli

AU - Danaher, Robert J.

AU - Stein, Daniel C.

N1 - Funding Information: This work was funded by a grant from the U.S. Navy to the University of Maryland through the University Research Initiative Program, grant No. N 00014-86K-0696.

PY - 1990/4/30

Y1 - 1990/4/30

N2 - A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.

AB - A type-II RM system, HjaI, was identified in the marine bacterium, Hyphomonas jannaschiana. the ENase recgonizes GATATC, and DNA fragments generated after cleavage with this enzyme contain blunt ends. A DNA fragment encoding these enzymes was cloned and expressed in Escherichia coli, although the level of expression of the cloned genes was low. DNA methylated by M·HjaI was not restricted by the Mcr or Mrr restriction systems of E. coli. Although H. jannaschiana is a marine bacterium isolated near the thermal vents on the floor of the Pacific Ocean, the biochemical properties of the ENase were similar to those of EcoRV, an isoschizomer isolated from E. coli.

KW - EcoRV isoschizomers

KW - Recombinant DNA

KW - enzymes

KW - hydrothermal vents methyltransferases

KW - marine bacterium

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JO - Gene

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Expression of closed restriction and modification genes, hjaIRM from jannaschiana in Escherichia coli

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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