TY - JOUR
T1 - Expression of multiple virus-derived resistance determinants in transgenic plants does not lead to additive resistance properties
AU - Maiti, Indu B.
AU - Von Lanken, Carol
AU - Hong, Yiling
AU - Dey, Nrisingha
AU - Hunt, Arthur G.
PY - 1999/7
Y1 - 1999/7
N2 - Plants can be protected against infection by potyviruses by expressing different portions of potyviral genomes as transgenes. This strategy has proven effective with several potyvirus genes, including the Nla, Nlb, and coat protein coding regions. Given the effectiveness of separate potyvirus coding regions as determinants of resistance, we tested the hypothesis that combinations of potyvirus coding regions would provide additively greater protection of plants against potyviruses. For this, we compared transgenic plant lines that expressed either the coat protein (CP) or the Nla+Nlb+coat protein (NNC) coding regions from tobaCCO vein mottling virus (TVMV). We found that plants that carry the NNC gene combination were invariably less resistant to TVMV than were lines that contain a CP gene alone. Additionally, we found that NNC lines displayed virtually no resistance to tobacco etch virus (TEV), in contrast to the CP lines. We conclude that combining more than one virus-derived resistance determinant in a single construct is detrimental to the production of virus-resistant plants.
AB - Plants can be protected against infection by potyviruses by expressing different portions of potyviral genomes as transgenes. This strategy has proven effective with several potyvirus genes, including the Nla, Nlb, and coat protein coding regions. Given the effectiveness of separate potyvirus coding regions as determinants of resistance, we tested the hypothesis that combinations of potyvirus coding regions would provide additively greater protection of plants against potyviruses. For this, we compared transgenic plant lines that expressed either the coat protein (CP) or the Nla+Nlb+coat protein (NNC) coding regions from tobaCCO vein mottling virus (TVMV). We found that plants that carry the NNC gene combination were invariably less resistant to TVMV than were lines that contain a CP gene alone. Additionally, we found that NNC lines displayed virtually no resistance to tobacco etch virus (TEV), in contrast to the CP lines. We conclude that combining more than one virus-derived resistance determinant in a single construct is detrimental to the production of virus-resistant plants.
KW - Pathogen-derived resistance
KW - Potyviruses
KW - Transgenic plants
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U2 - 10.1007/BF03263061
DO - 10.1007/BF03263061
M3 - Article
AN - SCOPUS:0032758711
SN - 0971-7811
VL - 8
SP - 67
EP - 73
JO - Journal of Plant Biochemistry and Biotechnology
JF - Journal of Plant Biochemistry and Biotechnology
IS - 2
ER -