Expression of splicing factors in human ovarian cancer

Dagmar C. Fischer, Kathleen Noack, Ingo B. Runnebaum, Dirk O. Watermann, Dirk G. Kieback, Stefan Stamm, Elmar Stickeler

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

Alternative splicing represents an important nuclear mechanism in the post-transcriptional regulation of gene expression, which is frequently altered during tumorigenesis. Previously, we have described marked changes in alternative splicing of the CD44 gene in ovarian and breast cancer. In the latter one we described also a specific induction of splicing factors during tumor development. Now we have focussed our studies on the expression profiles of splicing factors, including classical SR proteins, Tra2 and YB-1 in physiological and malignant ovarian tissues by RT-PCR and Western blot analysis. We detected changed expression pattern with higher levels of phosphorylated 30 kDa SR proteins as well as relatively high concentrations of hyperphosphorylated Tra2 protein isoforms in ovarian cancer. RT-PCR analysis revealed a marked induction of SC35 and ASF/SF2 as well as mRNA levels in malignant ovarian tissue. These results suggest gene-specific alterations of expression rather than a general induction of the splicing machinery. Together with previously performed functional studies of CD44 splicing these findings implicate that altered expression profiles of SR proteins, Tra2β and YB-1 might be responsible for the known changes of alternative CD44 splicing in ovarian cancer.

Original languageEnglish
Pages (from-to)1085-1090
Number of pages6
JournalOncology Reports
Volume11
Issue number5
DOIs
StatePublished - May 2004

Keywords

  • Alternative splicing
  • Ovarian cancer
  • SR proteins
  • Tra2
  • YB-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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