Expression of the Choline Acetyltransferase Gene Depends on Protein Kinase A Activity

Hiroyasu Inoue, Yi‐Ping ‐P Li, John A. Wagner, Louis B. Hersh

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Abstract: Choline acetyltransferase activity is barely detectable in a mutant pheochromocytoma PC12 cell line, A123.7, which is deficient in protein kinase A activity. Northern blot and polymerase chain reaction analyses showed that this mutant cell line has dramatically reduced levels of choline acetyltransferase mRNA, which correlates with the low level of enzyme activity. Transient transfection analysis was used to assess the functionality, in these cells, of an enhancer element and a cholinergic‐specific repressor element derived from the human choline acetyltransferase gene. The results show that the enhancer element is inactive in the protein kinase A‐deficient cell line. Cotransfection experiments with plasmids expressing the catalytic subunit of protein kinase A support this conclusion. These data indicate that protein kinase A regulates expression of the choline acetyltransferase gene at the transcriptional level by controlling the activity of an enhancer element.

Original languageEnglish
Pages (from-to)985-990
Number of pages6
JournalJournal of Neurochemistry
Issue number3
StatePublished - Mar 1995


  • Choline acetyltransferase
  • Enhancer
  • Gene regulation
  • Protein kinase A

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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