Expression of the tobacco β-1,3-glucanase gene, PR-2d, following induction of SAR with Peronospora tabacina

Deanna L. Funnell, Christopher B. Lawrence, Jeffrey F. Pedersen, Christopher L. Schardl

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic β-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to salicylic acid (SA), may be involved in regulation of PR-2d following induction of SAR with P. tabacina. This work provides evidence that regulation of PR-2 genes during P. tabacina-induced SAR may be similar to regulation of these genes during infection of N-gene tobacco by TMV or following exogenous application of SA, and provides further support for the role of SA in regulation of genes during P. tabacina-induced SAR.

Original languageEnglish
Pages (from-to)285-296
Number of pages12
JournalPhysiological and Molecular Plant Pathology
Volume65
Issue number6
DOIs
StatePublished - Dec 2004

Bibliographical note

Funding Information:
This work was supported by a grant from the Kentucky Tobacco Research Board to CLS. We are grateful to D. Klessig and J. Shah for providing N. tabacum lines transformed with PR-2d promoter constructs and to D. Klessig for providing a cDNA clone for the PR-2c gene. We thank E. Nuckles for valuable advice and discussions and R. French for editorial comments. We also thank W.T. Bass for maintenance of P. tabacina and for assistance with spray inoculations and J.D. Brown for maintenance of plants in the greenhouse. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of source.

Keywords

  • Downy mildew
  • Immunity
  • Nicotiana tabacum
  • Oomycete
  • Pathogenesis-related protein
  • Peronospora tabacina
  • Salicylic acid
  • Systemic acquired resistance
  • Tobacco blue mold
  • β 1,3-Glucanase

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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