Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Hiroaki Kusanagi; Akiko Hida; Kohtoku Satoh; Masaru Echizenya; Tetsuo Shimizu; Julie S. Pendergast; Shin Yamazaki; Kazuo Mishima

doi:10.1016/j.neures.2008.01.012

Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Hiroaki Kusanagi, Akiko Hida, Kohtoku Satoh, Masaru Echizenya, Tetsuo Shimizu, Julie S. Pendergast, Shin Yamazaki, Kazuo Mishima

Biology

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

Original language	English
Pages (from-to)	136-142
Number of pages	7
Journal	Neuroscience Research
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/j.neures.2008.01.012
State	Published - Jun 2008

Bibliographical note

Funding Information:
This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.

Keywords

Circadian
Clock genes
Expression profiles
Human peripheral blood mononuclear cells (PBMCs)
Period (Per)

ASJC Scopus subject areas

General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.neures.2008.01.012

Cite this

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title = "Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells",

abstract = "The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.",

keywords = "Circadian, Clock genes, Expression profiles, Human peripheral blood mononuclear cells (PBMCs), Period (Per)",

author = "Hiroaki Kusanagi and Akiko Hida and Kohtoku Satoh and Masaru Echizenya and Tetsuo Shimizu and Pendergast, {Julie S.} and Shin Yamazaki and Kazuo Mishima",

note = "Funding Information: This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.",

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doi = "10.1016/j.neures.2008.01.012",

language = "English",

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AU - Kusanagi, Hiroaki

AU - Hida, Akiko

AU - Satoh, Kohtoku

AU - Echizenya, Masaru

AU - Shimizu, Tetsuo

AU - Pendergast, Julie S.

AU - Yamazaki, Shin

AU - Mishima, Kazuo

N1 - Funding Information: This study was supported by Special Coordination Funds of the Ministry of Education, Culture, Sports, and Technology of Japan, and by a Grant-in-Aid for Cooperative Research from the Ministry of Health, Labour, and Welfare of Japan.

PY - 2008/6

Y1 - 2008/6

N2 - The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

AB - The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

KW - Circadian

KW - Clock genes

KW - Expression profiles

KW - Human peripheral blood mononuclear cells (PBMCs)

KW - Period (Per)

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Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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