Extended Middle Cerebral Artery Occlusion (MCAO) Model to Mirror Stroke Patients Undergoing Thrombectomy

Sarah J. Messmer, Kathleen E. Salmeron, Jacqueline A. Frank, Christopher J. McLouth, Douglas E. Lukins, Tyler C. Hammond, Ai Ling Lin, Justin F. Fraser, Keith R. Pennypacker

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Stroke remains a leading global cause of death and disability. In the last decade, the therapeutic window for mechanical thrombectomy has increased from a maximum of 6 to 24 h and beyond. While endovascular advancements have improved rates of recanalization, no post-stroke pharmacotherapeutics have been effective in enhancing neurorepair and recovery. New experimental models are needed to closer mimic the human patient. Our group has developed a model of transient 5-h occlusion in rats to mimic stroke patients undergoing thrombectomy. Our procedure was designed specifically in aged rats and was optimized based on sex in order to keep mortality and extent of injury consistent between aged male and female rats. This model uses a neurological assessment modeled after the NIH Stroke Scale. Finally, the potential for translation between our rat model of stroke and humans was assessed using comparative gene expression for key inflammatory genes. This model will be useful in the evaluation of therapeutic targets to develop adjuvant treatments for large vessel occlusion during the thrombectomy procedure.

Original languageEnglish
Pages (from-to)604-615
Number of pages12
JournalTranslational Stroke Research
Volume13
Issue number4
DOIs
StatePublished - Aug 2022

Bibliographical note

Funding Information:
Beverly Meacham (Senior Radiology Technologist) and David Powell (Assistant Professor) with The Magnetic Resonance Imaging and Spectroscopy Center. NIH Shared Instrumentation Program Grant for the 7T ClinScan. S10 (1S10RR029541-01).

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Aged
  • Behavior
  • MRI
  • Model
  • Rat

ASJC Scopus subject areas

  • Neuroscience (all)
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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