TY - JOUR
T1 - Extracellular vesicles released after cranial radiation
T2 - An insight into an early mechanism of brain injury
AU - Sukati, Suriyan
AU - Ho, Jenni
AU - Chaiswing, Luksana
AU - Sompol, Pradoldej
AU - Pandit, Harshul
AU - Wei, Wendy
AU - Izumi, Tadahide
AU - Chen, Quan
AU - Weiss, Heidi
AU - Noel, Teresa
AU - Bondada, Subbarao
AU - Allan Butterfield, D.
AU - St. Clair, Daret K.
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/5/1
Y1 - 2022/5/1
N2 - Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.
AB - Cranial radiation is important for treating both primary brain tumors and brain metastases. A potential delayed side effect of cranial radiation is neurocognitive function decline. Early detection of CNS injury might prevent further neuronal damage. Extracellular vesicles (EVs) have emerged as a potential diagnostic tool because of their unique membranous characteristics and cargos. We investigated whether EVs can be an early indicator of CNS injury by giving C57BJ/6 mice 10 Gy cranial IR. EVs were isolated from sera to quantify: 1) number of EVs using nanoparticle tracking analysis (NTA); 2) Glial fibrillary acidic protein (GFAP), an astrocyte marker; and 3) protein-bound 4-hydroxy-2-nonenal (HNE) adducts, an oxidative damage marker. Brain tissues were prepared for immunohistochemistry staining and protein immunoblotting. The results demonstrate: 1) increased GFAP levels (p < 0.05) in EVs, but not brain tissue, in the IR group; and 2) increased HNE-bound protein adduction levels (p < 0.05). The results support using EVs as an early indicator of cancer therapy-induced neuronal injury.
KW - Extracellular vesicles
KW - Lipid peroxidation
KW - Oxidative stress
KW - Radiation
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U2 - 10.1016/j.brainres.2022.147840
DO - 10.1016/j.brainres.2022.147840
M3 - Article
C2 - 35183524
AN - SCOPUS:85124732271
SN - 0006-8993
VL - 1782
JO - Brain Research
JF - Brain Research
M1 - 147840
ER -
