Facile chemoenzymatic strategies for the synthesis and utilization of S-adenosyl-L-methionine analogues

Shanteri Singh, Jianjun Zhang, Tyler D. Huber, Manjula Sunkara, Katherine Hurley, Randal D. Goff, Guojun Wang, Wen Zhang, Chunming Liu, Jürgen Rohr, Steven G. Van Lanen, Andrew J. Morris, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

A chemoenzymatic platform for the synthesis of S-adenosyl-L-methionine (SAM) analogues compatible with downstream SAM-utilizing enzymes is reported. Forty-four non-native S/Se-alkylated Met analogues were synthesized and applied to probing the substrate specificity of five diverse methionine adenosyltransferases (MATs). Human MAT II was among the most permissive of the MATs analyzed and enabled the chemoenzymatic synthesis of 29 non-native SAM analogues. As a proof of concept for the feasibility of natural product "alkylrandomization", a small set of differentially-alkylated indolocarbazole analogues was generated by using a coupled hMAT2-RebM system (RebM is the sugar C4′-O-methyltransferase that is involved in rebeccamycin biosynthesis). The ability to couple SAM synthesis and utilization in a single vessel circumvents issues associated with the rapid decomposition of SAM analogues and thereby opens the door for the further interrogation of a wide range of SAM utilizing enzymes. Mix and MATch: Methionine adenosyltransferase (MAT) was used to synthesize S-adenosylmethionine (SAM) analogues in a method directly compatible with downstream SAM-utilizing enzymes. As a proof of concept for the feasibility of natural product "alkylrandomization" by using this method, a coupled strategy in which MAT was applied in conjunction with the methyltransferase RebM was used to generate a small set of indolocarbazole analogues.

Original languageEnglish
Pages (from-to)3965-3969
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number15
DOIs
StatePublished - Apr 7 2014

Keywords

  • S-adenosylmethionine
  • alkylrandomization
  • biocatalysis
  • enzymes
  • methionine adenosyltransferase

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)

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