TY - JOUR
T1 - Facilitated long chain fatty acid uptake by adipocytes remains upregulated relative to BMI for more than a year after major bariatric surgical weight loss
AU - Ge, Fengxia
AU - Walewski, José L.
AU - Torghabeh, Mehyar Hefazi
AU - Lobdell, Harrison
AU - Hu, Chunguang
AU - Zhou, Shengli
AU - Dakin, Gregory
AU - Pomp, Alfons
AU - Bessler, Marc
AU - Schrope, Beth
AU - Ude-Welcome, Aku
AU - Inabnet, William B.
AU - Feng, Tianshu
AU - Carras-Terzian, Elektra
AU - Anglade, Dieunine
AU - Ebel, Faith E.
AU - Berk, Paul D.
N1 - Publisher Copyright:
© 2015 The Obesity Society.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Objective This study examined whether changes in adipocyte long chain fatty acid (LCFA) uptake kinetics explain the weight regain increasingly observed following bariatric surgery. Methods Three groups (10 patients each) were studied: patients without obesity (NO: BMI 24.2 ± 2.3 kg m-2); patients with obesity (O: BMI 49.8 ± 11.9); and patients classified as super-obese (SO: BMI 62.6 ± 2.8). NO patients underwent omental and subcutaneous fat biopsies during clinically indicated abdominal surgeries; O were biopsied during bariatric surgery, and SO during both a sleeve gastrectomy and at another bariatric operation 16 ± 2 months later, after losing 113 ± 13 lbs. Adipocyte sizes and [3H]-LCFA uptake kinetics were determined in all biopsies. Results Vmax for facilitated LCFA uptake by omental adipocytes increased exponentially from 5.1 ± 0.95 to 21.3 ± 3.20 to 68.7 ± 9.45 pmol/sec/50,000 cells in NO, O, and SO patients, respectively, correlating with BMI (r = 0.99, P < 0.001). Subcutaneous results were virtually identical. By the second operation, the mean BMI (SO patients) fell significantly (P < 0.01) to 44.4 ± 2.4 kg m-2, similar to the O group. However, Vmax (40.6 ± 11.5) in this weight-reduced group remained ∼2X that predicted from the BMI:Vmax regression among NO, O, and SO patients. Conclusions Facilitated adipocyte LCFA uptake remains significantly upregulated ≥1 year after bariatric surgery, possibly contributing to weight regain.
AB - Objective This study examined whether changes in adipocyte long chain fatty acid (LCFA) uptake kinetics explain the weight regain increasingly observed following bariatric surgery. Methods Three groups (10 patients each) were studied: patients without obesity (NO: BMI 24.2 ± 2.3 kg m-2); patients with obesity (O: BMI 49.8 ± 11.9); and patients classified as super-obese (SO: BMI 62.6 ± 2.8). NO patients underwent omental and subcutaneous fat biopsies during clinically indicated abdominal surgeries; O were biopsied during bariatric surgery, and SO during both a sleeve gastrectomy and at another bariatric operation 16 ± 2 months later, after losing 113 ± 13 lbs. Adipocyte sizes and [3H]-LCFA uptake kinetics were determined in all biopsies. Results Vmax for facilitated LCFA uptake by omental adipocytes increased exponentially from 5.1 ± 0.95 to 21.3 ± 3.20 to 68.7 ± 9.45 pmol/sec/50,000 cells in NO, O, and SO patients, respectively, correlating with BMI (r = 0.99, P < 0.001). Subcutaneous results were virtually identical. By the second operation, the mean BMI (SO patients) fell significantly (P < 0.01) to 44.4 ± 2.4 kg m-2, similar to the O group. However, Vmax (40.6 ± 11.5) in this weight-reduced group remained ∼2X that predicted from the BMI:Vmax regression among NO, O, and SO patients. Conclusions Facilitated adipocyte LCFA uptake remains significantly upregulated ≥1 year after bariatric surgery, possibly contributing to weight regain.
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U2 - 10.1002/oby.21249
DO - 10.1002/oby.21249
M3 - Article
C2 - 26584686
AN - SCOPUS:84951906493
VL - 24
SP - 113
EP - 122
IS - 1
ER -
