FACS to Identify Immune Subsets in Mouse Brain and Spleen

Mary K. Malone; Thomas A. Ujas; Katherine M. Cotter; Daimen R.S. Britsch; Jenny Lutshumba; Jadwiga Turchan-Cholewo; Ann M. Stowe

doi:10.1007/978-1-0716-2926-0_17

FACS to Identify Immune Subsets in Mouse Brain and Spleen

Mary K. Malone, Thomas A. Ujas, Katherine M. Cotter, Daimen R.S. Britsch, Jenny Lutshumba, Jadwiga Turchan-Cholewo, Ann M. Stowe

Research output: Contribution to journal › Article › peer-review

Abstract

Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.

Original language	English
Pages (from-to)	213-229
Number of pages	17
Journal	Methods in Molecular Biology
Volume	2616
DOIs	https://doi.org/10.1007/978-1-0716-2926-0_17
State	Published - 2023

Bibliographical note

Publisher Copyright:
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Brain
FACS
Flow cytometry
Lymphocytes
Single-cell suspension
Spleen
Stroke

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-2926-0_17

Cite this

@article{c46b0f5e2d574a07a6b8a9bf9ed44b3f,

title = "FACS to Identify Immune Subsets in Mouse Brain and Spleen",

abstract = "Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.",

keywords = "Brain, FACS, Flow cytometry, Lymphocytes, Single-cell suspension, Spleen, Stroke",

author = "Malone, {Mary K.} and Ujas, {Thomas A.} and Cotter, {Katherine M.} and Britsch, {Daimen R.S.} and Jenny Lutshumba and Jadwiga Turchan-Cholewo and Stowe, {Ann M.}",

note = "Publisher Copyright: {\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2023",

doi = "10.1007/978-1-0716-2926-0_17",

language = "English",

volume = "2616",

pages = "213--229",

}

TY - JOUR

T1 - FACS to Identify Immune Subsets in Mouse Brain and Spleen

AU - Malone, Mary K.

AU - Ujas, Thomas A.

AU - Cotter, Katherine M.

AU - Britsch, Daimen R.S.

AU - Lutshumba, Jenny

AU - Turchan-Cholewo, Jadwiga

AU - Stowe, Ann M.

PY - 2023

Y1 - 2023

N2 - Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.

AB - Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.

KW - Brain

KW - FACS

KW - Flow cytometry

KW - Lymphocytes

KW - Single-cell suspension

KW - Spleen

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=85147143769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85147143769&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2926-0_17

DO - 10.1007/978-1-0716-2926-0_17

M3 - Article

C2 - 36715938

AN - SCOPUS:85147143769

SN - 1064-3745

VL - 2616

SP - 213

EP - 229

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

FACS to Identify Immune Subsets in Mouse Brain and Spleen

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this