FACS to Identify Immune Subsets in Mouse Brain and Spleen

Mary K. Malone, Thomas A. Ujas, Katherine M. Cotter, Daimen R.S. Britsch, Jenny Lutshumba, Jadwiga Turchan-Cholewo, Ann M. Stowe

Research output: Contribution to journalArticlepeer-review


Flow cytometry enables the multi-parametric quantification of cell types, especially in immunophenotyping of unique immune cell subsets that can either contribute to or ameliorate pathology. For tissues to be used in such analyses, single-cell suspensions must be created. Here we describe protocols for preparing single-cell suspensions of mouse spleen and brain tissue, as well as the steps for fluorescently activated cell staining/sorting (FACS). Specifically, this protocol enables the isolation of lymphocytes for the study of immune responses during various diseases, such as long-term neuroinflammation following ischemic stroke.

Original languageEnglish
Pages (from-to)213-229
Number of pages17
JournalMethods in Molecular Biology
StatePublished - 2023

Bibliographical note

Publisher Copyright:
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • Brain
  • FACS
  • Flow cytometry
  • Lymphocytes
  • Single-cell suspension
  • Spleen
  • Stroke

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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