Falsely incompatible b-cell flow cytometry crossmatch after pronase treatment: A case report

J. D. Hart, C. T. Lutz, C. D. Jennings, J. R. May, K. Nelson, S. Jacobs, C. W. Hoopes

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

This report presents a falsely incompatible B cell crossmatch by flow cytometry in a lung transplant recipient. The patient was a 35-year-old Caucasian male with end-stage lung disease secondary to cystic fibrosis whose pretransplantation serologic workup did not disclose the presence of anti-HLA class II antibodies by single antigen bead testing. Unexpectedly, crossmatch of recipient sera with pronase-treated donor lymphocytes resulted in antibody binding to B cells only. The positive reactivity was reproducible in pronase-treated autologous B cells. Recipient sera did not react with nontreated donor or autologous lymphocytes. Herein, we describe our approach to this unexpected crossmatch result and consider the implications of false-positive crossmatch results on transplantation.

Original languageEnglish
Pages (from-to)831-833
Number of pages3
JournalTransplantation Proceedings
Volume47
Issue number3
DOIs
StatePublished - Apr 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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