TY - JOUR
T1 - Falsely incompatible b-cell flow cytometry crossmatch after pronase treatment
T2 - A case report
AU - Hart, J. D.
AU - Lutz, C. T.
AU - Jennings, C. D.
AU - May, J. R.
AU - Nelson, K.
AU - Jacobs, S.
AU - Hoopes, C. W.
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - This report presents a falsely incompatible B cell crossmatch by flow cytometry in a lung transplant recipient. The patient was a 35-year-old Caucasian male with end-stage lung disease secondary to cystic fibrosis whose pretransplantation serologic workup did not disclose the presence of anti-HLA class II antibodies by single antigen bead testing. Unexpectedly, crossmatch of recipient sera with pronase-treated donor lymphocytes resulted in antibody binding to B cells only. The positive reactivity was reproducible in pronase-treated autologous B cells. Recipient sera did not react with nontreated donor or autologous lymphocytes. Herein, we describe our approach to this unexpected crossmatch result and consider the implications of false-positive crossmatch results on transplantation.
AB - This report presents a falsely incompatible B cell crossmatch by flow cytometry in a lung transplant recipient. The patient was a 35-year-old Caucasian male with end-stage lung disease secondary to cystic fibrosis whose pretransplantation serologic workup did not disclose the presence of anti-HLA class II antibodies by single antigen bead testing. Unexpectedly, crossmatch of recipient sera with pronase-treated donor lymphocytes resulted in antibody binding to B cells only. The positive reactivity was reproducible in pronase-treated autologous B cells. Recipient sera did not react with nontreated donor or autologous lymphocytes. Herein, we describe our approach to this unexpected crossmatch result and consider the implications of false-positive crossmatch results on transplantation.
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U2 - 10.1016/j.transproceed.2014.12.022
DO - 10.1016/j.transproceed.2014.12.022
M3 - Article
C2 - 25891741
AN - SCOPUS:84928338645
SN - 0041-1345
VL - 47
SP - 831
EP - 833
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 3
ER -