Farnesyl diphosphate analogues with Aryl moieties are efficient alternate substrates for protein farnesyltransferase

Thangaiah Subramanian, June E. Pais, Suxia Liu, Jerry M. Troutman, Yuta Suzuki, Karunai Leela Subramanian, Carol A. Fierke, Douglas A. Andres, H. Peter Spielmann

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Farnesylation is an important post-translational modification essential for the proper localization and function of many proteins. Transfer of the farnesyl group from farnesyl diphosphate (FPP) to proteins is catalyzed by protein farnesyltransferase (FTase). We employed a library of FPP analogues with a range of aryl groups substituting for individual isoprene moieties to examine some of the structural and electronic properties of the transfer of an analogue to the peptide catalyzed by FTase. Analysis of steady-state kinetics for modification of peptide substrates revealed that the multiple-turnover activity depends on the analogue structure. Analogues in which the first isoprene is replaced with a benzyl group and an analogue in which each isoprene is replaced with an aryl group are good substrates. In sharp contrast with the steady-state reaction, the single-turnover rate constant for dansyl-GCVLS alkylation was found to be the same for all analogues, despite the increased chemical reactivity of the benzyl analogues and the increased steric bulk of other analogues. However, the single-turnover rate constant for alkylation does depend on the Ca 1a2X peptide sequence. These results suggest that the isoprenoid transition-state conformation is preferred over the inactive E·FPP·Ca1a2X ternary complex conformation. Furthermore, these data suggest that the farnesyl binding site in the exit groove may be significantly more selective for the farnesyl diphosphate substrate than the active site binding pocket and therefore might be a useful site for the design of novel inhibitors.

Original languageEnglish
Pages (from-to)8307-8319
Number of pages13
JournalBiochemistry
Volume51
Issue number41
DOIs
StatePublished - Oct 16 2012

ASJC Scopus subject areas

  • Biochemistry

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