Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

W. S. Grayburn; G. B. Collins; D. F. Hildebrand

doi:10.1038/nbt0692-675

Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

W. S. Grayburn, G. B. Collins, D. F. Hildebrand

Plant and Soil Sciences

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38 Scopus citations

Abstract

Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

Original language	English
Pages (from-to)	675-678
Number of pages	4
Journal	Bio/Technology
Volume	10
Issue number	6
DOIs	https://doi.org/10.1038/nbt0692-675
State	Published - Jun 1992

ASJC Scopus subject areas

Biotechnology

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abstract = "Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.",

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AB - Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

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Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

Abstract

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