Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

W. S. Grayburn, G. B. Collins, D. F. Hildebrand

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
Issue number6
StatePublished - Jun 1992

ASJC Scopus subject areas

  • Biotechnology


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