Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury

Allyson L. Huttinger; Debra G. Wheeler; Surya Gnyawali; David Dornbos; Juliana M. Layzer; Nicholas Venetos; Spencer Talentino; Nicholas J. Musgrave; Cheyenne Jones; Camille Bratton; Matthew E. Joseph; Chandan Sen; Bruce A. Sullenger; Shahid M. Nimjee

doi:10.3791/57981

Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury

Allyson L. Huttinger
, Debra G. Wheeler
, Surya Gnyawali
, David Dornbos
, Juliana M. Layzer
, Nicholas Venetos
, Spencer Talentino
, Nicholas J. Musgrave
, Cheyenne Jones
, Camille Bratton
, Matthew E. Joseph
, Chandan Sen
, Bruce A. Sullenger
, Shahid M. Nimjee

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

Original language	English
Article number	e57981
Journal	Journal of Visualized Experiments
Volume	2018
Issue number	139
DOIs	https://doi.org/10.3791/57981
State	Published - Sep 7 2018

Bibliographical note

Publisher Copyright:
© 2018, Journal of Visualized Experiments. All rights reserved.

Funding

We would like to thank the Center for Cognitive and Behavioral Brain Imaging at The Ohio State University for their financial and scientific support to develop and perform canine magnetic resonance imaging.

Funders
Ohio Water Resources Center, Ohio State University

Keywords

Angiography
Animal model
Canine
Carotid artery
Doppler flow meter
Ferric chloride
Issue 139
Magnetic resonance imaging
Medicine
Thrombosis
Vascular injury

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

Access to Document

10.3791/57981

Cite this

Huttinger, A. L., Wheeler, D. G., Gnyawali, S., Dornbos, D., Layzer, J. M., Venetos, N., Talentino, S., Musgrave, N. J., Jones, C., Bratton, C., Joseph, M. E., Sen, C., Sullenger, B. A., & Nimjee, S. M. (2018). Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury. Journal of Visualized Experiments, 2018(139), Article e57981. https://doi.org/10.3791/57981

@article{071c71b8a83c49d8bb2b8a53356a725e,

title = "Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury",

abstract = "Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to \textasciitilde{}13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.",

keywords = "Angiography, Animal model, Canine, Carotid artery, Doppler flow meter, Ferric chloride, Issue 139, Magnetic resonance imaging, Medicine, Thrombosis, Vascular injury",

author = "Huttinger, \{Allyson L.\} and Wheeler, \{Debra G.\} and Surya Gnyawali and David Dornbos and Layzer, \{Juliana M.\} and Nicholas Venetos and Spencer Talentino and Musgrave, \{Nicholas J.\} and Cheyenne Jones and Camille Bratton and Joseph, \{Matthew E.\} and Chandan Sen and Sullenger, \{Bruce A.\} and Nimjee, \{Shahid M.\}",

year = "2018",

month = sep,

day = "7",

doi = "10.3791/57981",

language = "English",

volume = "2018",

number = "139",

}

Huttinger, AL, Wheeler, DG, Gnyawali, S, Dornbos, D, Layzer, JM, Venetos, N, Talentino, S, Musgrave, NJ, Jones, C, Bratton, C, Joseph, ME, Sen, C, Sullenger, BA & Nimjee, SM 2018, 'Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury', Journal of Visualized Experiments, vol. 2018, no. 139, e57981. https://doi.org/10.3791/57981

TY - JOUR

T1 - Ferric chloride-induced canine carotid artery thrombosis

T2 - A large animal model of vascular injury

AU - Huttinger, Allyson L.

AU - Wheeler, Debra G.

AU - Gnyawali, Surya

AU - Dornbos, David

AU - Layzer, Juliana M.

AU - Venetos, Nicholas

AU - Talentino, Spencer

AU - Musgrave, Nicholas J.

AU - Jones, Cheyenne

AU - Bratton, Camille

AU - Joseph, Matthew E.

AU - Sen, Chandan

AU - Sullenger, Bruce A.

AU - Nimjee, Shahid M.

PY - 2018/9/7

Y1 - 2018/9/7

N2 - Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

AB - Occlusive arterial thrombosis leading to cerebral ischemic stroke and myocardial infarction contributes to ~13 million deaths every year globally. Here, we have translated a vascular injury model from a small animal into a large animal (canine), with slight modifications that can be used for pre-clinical screening of prophylactic and thrombolytic agents. In addition to the surgical methods, the modified protocol describes the step-by-step methods to assess carotid artery canalization by angiography, detailed instructions to process both the brain and carotid artery for histological analysis to verify carotid canalization and cerebral hemorrhage, and specific parameters to complete an assessment of downstream thromboembolic events by utilizing magnetic resonance imaging (MRI). In addition, specific procedural changes from the previously well-established small animal model necessary to translate into a large animal (canine) vascular injury are discussed.

KW - Angiography

KW - Animal model

KW - Canine

KW - Carotid artery

KW - Doppler flow meter

KW - Ferric chloride

KW - Issue 139

KW - Magnetic resonance imaging

KW - Medicine

KW - Thrombosis

KW - Vascular injury

UR - https://www.scopus.com/pages/publications/85054385104

UR - https://www.scopus.com/pages/publications/85054385104#tab=citedBy

U2 - 10.3791/57981

DO - 10.3791/57981

M3 - Article

C2 - 30247470

AN - SCOPUS:85054385104

SN - 1940-087X

VL - 2018

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 139

M1 - e57981

ER -

Ferric chloride-induced canine carotid artery thrombosis: A large animal model of vascular injury

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this