Fertilization-independent cell-fusion between the synergid and central cell in the polycomb mutant

Kazuki Motomura, Frédéric Berger, Tomokazu Kawashima, Tetsu Kinoshita, Tetsuya Higashiyama, Daisuke Maruyama

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


In flowering plants, fertilization of the central cell gives rise to an embryo-nourishing endosperm. Recently, we reported that the endosperm absorbs the adjacent synergid cell through a cell-fusion, terminating the pollen tube guidance by a rapid inactivation of the synergid cell. Although this synergid-endosperm fusion (SE fusion) initiates soon after fertilization, it was still unknown whether the triggers of SE fusion are stimuli during fertilization or other seed developmental processes. To further dissect out the SE fusion process, we investigated the SE fusion in an Arabidopsis mutant defective for MULTICOPY SUPPRESSOR OF IRA1 (MSI1), a subunit of the polycomb repressive complex 2 (PRC2). The mutant msi1 develops autonomous endosperm without fertilization. Time-lapse imaging revealed a rapid efflux of the synergid contents during the autonomous endosperm development, indicating that the initiation of SE fusion is under the control of some of the events triggered by fertilization of the central cell distinct from the discharge of pollen tube contents and plasma membrane fusion.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalCell Structure and Function
Issue number2
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 by Japan Society for Cell Biology.


  • Double fertilization
  • Endosperm
  • Polycomb repressive complex2 (PRC2)
  • Polytubey block
  • Synergid endosperm fusion

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology


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