Ferulic acid antioxidant protection against hydroxyl and peroxyl radical oxidation in synaptosomal and neuronal cell culture systems in vitro: Structure-activity studies

Jaroslaw Kanski, Marina Aksenova, Antonia Stoyanova, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

427 Scopus citations

Abstract

In this study, free radical scavenging abilities of ferulic acid in relation to its structural characteristics were evaluated in solution, cultured neurons, and synaptosomal systems exposed to hydroxyl and peroxyl radicals. Cultured neuronal cells exposed to the peroxyl radical initiator AAPH die in a dose-response manner and show elevated levels of protein carbonyls. The presence of ferulic acid or similar phenolic compounds, however, greatly reduces free radical damage in neuronal cell systems without causing cell death by themselves. In addition, synaptosomal membrane systems exposed to oxidative stress by hydroxyl and peroxyl radical generators show elevated levels of oxidation as indexed by protein oxidation, lipid peroxidation, and ROS measurement. Ferulic acid greatly attenuates these changes, and its effects are far more potent than those obtained for vanillic, coumaric, and cinnamic acid treatments. Moreover, ferulic acid protects against free radical mediated changes in conformation of synaptosomal membrane proteins as monitored by EPR spin labeling techniques. The results presented in this study suggest the importance of naturally occurring antioxidants such as ferulic acid in therapeutic intervention methodology against neurodegenerative disorders such as Alzheimer's disease in which oxidative stress is implicated.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalJournal of Nutritional Biochemistry
Volume13
Issue number5
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
This work was supported in part by grants from NIH to D.A.B. [AG-05119; AG-10836; AG-12423].

Keywords

  • Antioxidants
  • DCF fluorescence
  • EPR
  • Ferulic acid
  • Neuronal cultures
  • Oxidative stress
  • Reactive oxygen species
  • Synaptosomal membranes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

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