Fetal-fluid proteome analyses in late-term healthy pregnant mares and in mares with experimentally induced ascending placentitis

Igor F. Canisso, Shavahn Loux, Kirsten E. Scoggin, Edward L. Squires, Mats H. Troedsson, Barry A. Ball

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Characterisation of fetal fluids in healthy and disease states of pregnant mares can help to unravel the pathophysiology and to identify putative markers of disease. Thus, this study aimed to compare the protein composition of: (1) amniotic and allantoic fluids of healthy mares obtained immediately after euthanasia and (2) allantoic fluid harvested via centesis before and after experimental induction of placentitis via transcervical inoculation of Streptococcus equi ssp zooepidemicus in healthy mares. Fetal fluids were analysed with a high-throughput proteomic technique after in-gel digestion. Statistical comparisons were performed following normalisation of peptide spectral match. Global normalisation was performed to calculate relative expression. There were 112 unique proteins present in both allantoic and amniotic fluids. There were 13 and 29 proteins defined as amniotic- or allantoic-specific respectively that were present in at least two fluid samples. Another 26 proteins were present in both amniotic and allantoic fluids. Panther DB functional classification grouped fetal-fluid proteins as transfer carriers, signalling molecules, receptors, immunity, hydrolase, enzymes, membrane traffic, cytoskeleton, cell adhesion, calcium binding and extracellular matrix. Experimentally induced placentitis resulted in 10 proteins being upregulated and 10 downregulated in allantoic fluid. Newly identified proteins and changes in the fetal-fluid proteome provide clues about the physiology of pregnancy and pathogenesis of placentitis.

Original languageEnglish
Pages (from-to)1486-1496
Number of pages11
JournalReproduction, Fertility and Development
Volume31
Issue number9
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
Funds were provided by the Kentucky Thoroughbred Association/Kentucky Thoroughbred Breeders and Owners and by the University of Kentucky (Department of Veterinary Science, Albert G. Clay Endowment and Geoffrey Hughes Fellowship). The authors would like to thank Michelle Wynn, Jessalyn Walter, Gabriel Davolli, Sydney Hughes and Carol Beach for their assistance during this study. The mass spectrometric analysis was performed at the University of Kentucky Proteomics Core Facility. This core facility is supported in part by funds from the Office of the Vice President for Research.

Publisher Copyright:
© 2019 CSIRO.

Keywords

  • horse
  • pregnancy disease
  • pregnancy physiology
  • protein ontology

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

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