Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development

Sruthi Purushothaman, Ahmed Elewa, Ashley W. Seifert

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Although decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: Their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how Sonic hedgehog (Shh) and Fibroblast growth factor (Fgf) signaling regulate limb development in the axolotl. We found that Shh-expressing cells contributed to the most posterior digit, and that inhibiting Shh-signaling inhibited Fgf8 expression, anteroposterior patterning, and distal cell proliferation. In addition to lack of a morphological AER, we found that salamander limbs also lack a molecular AER. We found that amniote and anuran AER-specific Fgfs and their cognate receptors were expressed entirely in the mesenchyme. Broad inhibition of Fgf-signaling demonstrated that this pathway regulates cell proliferation across all three limb axes, in contrast to anurans and amniotes where Fgf-signaling regulates cell survival and proximodistal patterning.

Original languageEnglish
Article numbere48507
JournaleLife
Volume8
DOIs
StatePublished - Sep 2019

Bibliographical note

Funding Information:
National Science Foundation, IOS-1353713.

Publisher Copyright:
© Purushothaman et al.

ASJC Scopus subject areas

  • Neuroscience (all)
  • Immunology and Microbiology (all)
  • Biochemistry, Genetics and Molecular Biology (all)

Fingerprint

Dive into the research topics of 'Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development'. Together they form a unique fingerprint.

Cite this