Flow cytometry and K-mer analysis estimates of the genome sizes of Bemisia tabaci B and Q (Hemiptera: Aleyrodidae)

Li T. Guo, Shao L. Wang, Qing J. Wu, Xu G. Zhou, Wen Xie, You J. Zhang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The genome sizes of the B- and Q-types of the whitefly Bemisia tabaci (Gennnadius) were estimated using flow cytometry (Drosophila melanogaster as the DNA reference standard and propidium iodide (PI) as the fluorochrome) and k-mer analysis. For flow cytometry, the mean nuclear DNA content was 0.686 pg for B-type males, 1.392 pg for B-type females, 0.680 pg for Q-type males, and 1.306 pg for Q-type females. Based on the relationship between DNA content and genome size (1 pg DNA = 980 Mbp), the haploid genome size of B. tabaci ranged from 640 to 682 Mbp. For k-mer analysis, genome size of B-type by two methods were consistent highly, but the k-mer depth distribution graph of Q-type was not enough perfect and the genome size was estimated about 60 M larger than its flow cytometry result. These results corroborate previous reports of genome size based on karyotype analysis and chromosome counting. However, these estimates differ from previous flow cytometry estimates, probably because of differences in the DNA reference standard and dyeing time, which were superior in the current study. For Q-type genome size difference by two method, some discussion were also stated, and all these results represent a useful foundation for B. tabaci genomics research.

Original languageEnglish
Article number144
JournalFrontiers in Physiology
Volume6
Issue numberMAY
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 Guo, Wang, Wu, Zhou, Xie and Zhang.

Keywords

  • Bemisia tabaci
  • Flow cytometry
  • Genome size
  • K-mer analysis
  • Nuclear DNA content

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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