Abstract
Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
Original language | English |
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Pages (from-to) | E150-E154 |
Journal | Anesthesia and Analgesia |
Volume | 129 |
Issue number | 5 |
DOIs | |
State | Published - Nov 1 2019 |
Bibliographical note
Funding Information:Accepted for publication March 11, 2019. Funding: M.B. acknowledges support from a DREAM Innovation grant from the Duke Anesthesiology department, support from National Institutes of Health T32-GM08600, an International Anesthesia Research Society Mentored Research Award, National Institutes of Health R03-AG050918, and National Institutes of Health K76-AG057022, a Jahnigen Scholars Fellowship award, a small project grant from the American Geriatrics Society, a William L. Young Neuroscience Research Award from the Society for Neuroscience in Anesthesiology and Critical Care, and additional support from the National Institute on Aging (P30-AG028716). D.M.M. acknowledges support from 1R01DA043241. Q.J.Q. acknowledges support from National Institutes of Health T32-GM08600 and a Society of Cardiovascular Anesthesiologists/International Anesthesia Research Society starter grant. J.P.M. acknowledges support from National Institutes of Health R01-HL130443. K.J.W. acknowledges support from National Institutes of Health P30-AI064518.
Publisher Copyright:
© 2019 International Anesthesia Research Society.
ASJC Scopus subject areas
- Anesthesiology and Pain Medicine