Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study

Miles Berger; David M. Murdoch; Janet S. Staats; Cliburn Chan; Jake P. Thomas; Grant E. Garrigues; Jeffrey N. Browndyke; Mary Cooter; Quintin J. Quinones; Joseph P. Mathew; Kent J. Weinhold; Cindy L. Amundsen; Shahrukh Bengali; Brian E. Brigman; W. Michael Bullock; Jessica Carter; Joseph Chapman; Vanessa Cheong Yee Ching; Harvey J. Cohen; Brian Colin; Thomas A. D'Amico; Michael J. Devinney; James K. Deorio; Tressa Ellet; Ramon M. Esclamado; Michael N. Ferrandino; Jeffrey Gadsden; Jason Guercio; Ashraf Habib; David H. Harpole; Mathew G. Hartwig; Ehimemen Iboaya; Brant A. Inman; Anver Khan; Sandhya Lagoo-Deenadayalan; Paula S. Lee; Walter T. Lee; John Lemm; Howard Levinson; Christopher Mantyh; David L. McDonagh; John Migaly; Suhail K. Mithani; Eugene Moretti; Judd W. Moul; Mark F. Newman; Katherine Ni; Brian Ohlendorf; Alexander Perez; Andrew C. Peterson; Vikram Ponussamy; Glenn M. Preminger; Cary N. Robertson; Sanziana A. Roman; Scott Runyon; Aaron Sandler; Randall P. Scheri; S. Kendall Smith; Leonard Talbot; Julie K.M. Thacker; Betty C. Tong; Alexander Tu; Steven N. Vaslef; Nathan Waldron; Xueyuan Wang; Heather Whitson; Victoria Wickenheisser; Christopher Young

doi:10.1213/ANE.0000000000004179

Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study

Miles Berger, David M. Murdoch, Janet S. Staats, Cliburn Chan, Jake P. Thomas, Grant E. Garrigues, Jeffrey N. Browndyke, Mary Cooter, Quintin J. Quinones, Joseph P. Mathew, Kent J. Weinhold, Cindy L. Amundsen, Shahrukh Bengali, Brian E. Brigman, W. Michael Bullock, Jessica Carter, Joseph Chapman, Vanessa Cheong Yee Ching, Harvey J. Cohen, Brian ColinThomas A. D'Amico, Michael J. Devinney, James K. Deorio, Tressa Ellet, Ramon M. Esclamado, Michael N. Ferrandino, Jeffrey Gadsden, Jason Guercio, Ashraf Habib, David H. Harpole, Mathew G. Hartwig, Ehimemen Iboaya, Brant A. Inman, Anver Khan, Sandhya Lagoo-Deenadayalan, Paula S. Lee, Walter T. Lee, John Lemm, Howard Levinson, Christopher Mantyh, David L. McDonagh, John Migaly, Suhail K. Mithani, Eugene Moretti, Judd W. Moul, Mark F. Newman, Katherine Ni, Brian Ohlendorf, Alexander Perez, Andrew C. Peterson, Vikram Ponussamy, Glenn M. Preminger, Cary N. Robertson, Sanziana A. Roman, Scott Runyon, Aaron Sandler, Randall P. Scheri, S. Kendall Smith, Leonard Talbot, Julie K.M. Thacker, Betty C. Tong, Alexander Tu, Steven N. Vaslef, Nathan Waldron, Xueyuan Wang, Heather Whitson, Victoria Wickenheisser, Christopher Young

Anesthesiology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

Original language	English
Pages (from-to)	E150-E154
Journal	Anesthesia and Analgesia
Volume	129
Issue number	5
DOIs	https://doi.org/10.1213/ANE.0000000000004179
State	Published - Nov 1 2019

Bibliographical note

Publisher Copyright:
© 2019 International Anesthesia Research Society.

Funding

Accepted for publication March 11, 2019. Funding: M.B. acknowledges support from a DREAM Innovation grant from the Duke Anesthesiology department, support from National Institutes of Health T32-GM08600, an International Anesthesia Research Society Mentored Research Award, National Institutes of Health R03-AG050918, and National Institutes of Health K76-AG057022, a Jahnigen Scholars Fellowship award, a small project grant from the American Geriatrics Society, a William L. Young Neuroscience Research Award from the Society for Neuroscience in Anesthesiology and Critical Care, and additional support from the National Institute on Aging (P30-AG028716). D.M.M. acknowledges support from 1R01DA043241. Q.J.Q. acknowledges support from National Institutes of Health T32-GM08600 and a Society of Cardiovascular Anesthesiologists/International Anesthesia Research Society starter grant. J.P.M. acknowledges support from National Institutes of Health R01-HL130443. K.J.W. acknowledges support from National Institutes of Health P30-AI064518.

Funders	Funder number
Duke Anesthesiology Department
Society of Cardiovascular Anesthesiologists/International Anesthesia Research Society	P30-AI064518, R01-HL130443
National Institutes of Health (NIH)	K76-AG057022, T32-GM08600
National Institutes of Health (NIH)
National Institute on Aging	P30-AG028716, R03AG050918, 1R01DA043241
National Institute on Aging
American Geriatrics Society
Society for Neuroscience in Anesthesiology and Critical Care

ASJC Scopus subject areas

Anesthesiology and Pain Medicine

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10.1213/ANE.0000000000004179

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Berger, M., Murdoch, D. M., Staats, J. S., Chan, C., Thomas, J. P., Garrigues, G. E., Browndyke, J. N., Cooter, M., Quinones, Q. J., Mathew, J. P., Weinhold, K. J., Amundsen, C. L., Bengali, S., Brigman, B. E., Bullock, W. M., Carter, J., Chapman, J., Cheong Yee Ching, V., Cohen, H. J., ... Young, C. (2019). Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study. Anesthesia and Analgesia, 129(5), E150-E154. https://doi.org/10.1213/ANE.0000000000004179

@article{f8bbabc123814491a3674b064886488d,

title = "Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study",

abstract = "Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.",

author = "Miles Berger and Murdoch, \{David M.\} and Staats, \{Janet S.\} and Cliburn Chan and Thomas, \{Jake P.\} and Garrigues, \{Grant E.\} and Browndyke, \{Jeffrey N.\} and Mary Cooter and Quinones, \{Quintin J.\} and Mathew, \{Joseph P.\} and Weinhold, \{Kent J.\} and Amundsen, \{Cindy L.\} and Shahrukh Bengali and Brigman, \{Brian E.\} and Bullock, \{W. Michael\} and Jessica Carter and Joseph Chapman and \{Cheong Yee Ching\}, Vanessa and Cohen, \{Harvey J.\} and Brian Colin and D'Amico, \{Thomas A.\} and Devinney, \{Michael J.\} and Deorio, \{James K.\} and Tressa Ellet and Esclamado, \{Ramon M.\} and Ferrandino, \{Michael N.\} and Jeffrey Gadsden and Jason Guercio and Ashraf Habib and Harpole, \{David H.\} and Hartwig, \{Mathew G.\} and Ehimemen Iboaya and Inman, \{Brant A.\} and Anver Khan and Sandhya Lagoo-Deenadayalan and Lee, \{Paula S.\} and Lee, \{Walter T.\} and John Lemm and Howard Levinson and Christopher Mantyh and McDonagh, \{David L.\} and John Migaly and Mithani, \{Suhail K.\} and Eugene Moretti and Moul, \{Judd W.\} and Newman, \{Mark F.\} and Katherine Ni and Brian Ohlendorf and Alexander Perez and Peterson, \{Andrew C.\} and Vikram Ponussamy and Preminger, \{Glenn M.\} and Robertson, \{Cary N.\} and Roman, \{Sanziana A.\} and Scott Runyon and Aaron Sandler and Scheri, \{Randall P.\} and Smith, \{S. Kendall\} and Leonard Talbot and Thacker, \{Julie K.M.\} and Tong, \{Betty C.\} and Alexander Tu and Vaslef, \{Steven N.\} and Nathan Waldron and Xueyuan Wang and Heather Whitson and Victoria Wickenheisser and Christopher Young",

note = "Publisher Copyright: {\textcopyright} 2019 International Anesthesia Research Society.",

year = "2019",

month = nov,

day = "1",

doi = "10.1213/ANE.0000000000004179",

language = "English",

volume = "129",

pages = "E150--E154",

number = "5",

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Berger, M, Murdoch, DM, Staats, JS, Chan, C, Thomas, JP, Garrigues, GE, Browndyke, JN, Cooter, M, Quinones, QJ, Mathew, JP, Weinhold, KJ, Amundsen, CL, Bengali, S, Brigman, BE, Bullock, WM, Carter, J, Chapman, J, Cheong Yee Ching, V, Cohen, HJ, Colin, B, D'Amico, TA, Devinney, MJ, Deorio, JK, Ellet, T, Esclamado, RM, Ferrandino, MN, Gadsden, J, Guercio, J, Habib, A, Harpole, DH, Hartwig, MG, Iboaya, E, Inman, BA, Khan, A, Lagoo-Deenadayalan, S, Lee, PS, Lee, WT, Lemm, J, Levinson, H, Mantyh, C, McDonagh, DL, Migaly, J, Mithani, SK, Moretti, E, Moul, JW, Newman, MF, Ni, K, Ohlendorf, B, Perez, A, Peterson, AC, Ponussamy, V, Preminger, GM, Robertson, CN, Roman, SA, Runyon, S, Sandler, A, Scheri, RP, Smith, SK, Talbot, L, Thacker, JKM, Tong, BC, Tu, A, Vaslef, SN, Waldron, N, Wang, X, Whitson, H, Wickenheisser, V & Young, C 2019, 'Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study', Anesthesia and Analgesia, vol. 129, no. 5, pp. E150-E154. https://doi.org/10.1213/ANE.0000000000004179

TY - JOUR

T1 - Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction

T2 - A Pilot Study

AU - Berger, Miles

AU - Murdoch, David M.

AU - Staats, Janet S.

AU - Chan, Cliburn

AU - Thomas, Jake P.

AU - Garrigues, Grant E.

AU - Browndyke, Jeffrey N.

AU - Cooter, Mary

AU - Quinones, Quintin J.

AU - Mathew, Joseph P.

AU - Weinhold, Kent J.

AU - Amundsen, Cindy L.

AU - Bengali, Shahrukh

AU - Brigman, Brian E.

AU - Bullock, W. Michael

AU - Carter, Jessica

AU - Chapman, Joseph

AU - Cheong Yee Ching, Vanessa

AU - Cohen, Harvey J.

AU - Colin, Brian

AU - D'Amico, Thomas A.

AU - Devinney, Michael J.

AU - Deorio, James K.

AU - Ellet, Tressa

AU - Esclamado, Ramon M.

AU - Ferrandino, Michael N.

AU - Gadsden, Jeffrey

AU - Guercio, Jason

AU - Habib, Ashraf

AU - Harpole, David H.

AU - Hartwig, Mathew G.

AU - Iboaya, Ehimemen

AU - Inman, Brant A.

AU - Khan, Anver

AU - Lagoo-Deenadayalan, Sandhya

AU - Lee, Paula S.

AU - Lee, Walter T.

AU - Lemm, John

AU - Levinson, Howard

AU - Mantyh, Christopher

AU - McDonagh, David L.

AU - Migaly, John

AU - Mithani, Suhail K.

AU - Moretti, Eugene

AU - Moul, Judd W.

AU - Newman, Mark F.

AU - Ni, Katherine

AU - Ohlendorf, Brian

AU - Perez, Alexander

AU - Peterson, Andrew C.

AU - Ponussamy, Vikram

AU - Preminger, Glenn M.

AU - Robertson, Cary N.

AU - Roman, Sanziana A.

AU - Runyon, Scott

AU - Sandler, Aaron

AU - Scheri, Randall P.

AU - Smith, S. Kendall

AU - Talbot, Leonard

AU - Thacker, Julie K.M.

AU - Tong, Betty C.

AU - Tu, Alexander

AU - Vaslef, Steven N.

AU - Waldron, Nathan

AU - Wang, Xueyuan

AU - Whitson, Heather

AU - Wickenheisser, Victoria

AU - Young, Christopher

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

AB - Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.

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UR - http://www.scopus.com/inward/citedby.url?scp=85072154227&partnerID=8YFLogxK

U2 - 10.1213/ANE.0000000000004179

DO - 10.1213/ANE.0000000000004179

M3 - Article

C2 - 31085945

AN - SCOPUS:85072154227

SN - 0003-2999

VL - 129

SP - E150-E154

JO - Anesthesia and Analgesia

JF - Anesthesia and Analgesia

IS - 5

ER -

Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients with Postoperative Cognitive Dysfunction: A Pilot Study

Abstract

Bibliographical note

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ASJC Scopus subject areas

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