TY - JOUR
T1 - Follicular characteristics associated with viable pregnancy after in vitro fertilization in humans
AU - Nayudu, P. L.
AU - Gook, D. A.
AU - Lopata, A.
AU - Sheather, S. J.
AU - Lloyd‐Smith, C. W.
AU - Cadusch, P.
AU - Johnston, W. I.H.
PY - 1987/9
Y1 - 1987/9
N2 - The aim of this study has been the development of a noninvasive method of predicting the pregnancy potential of human oocytes and embryos intended for in vitro fertilization and embryo replacement. A multifactorial system which distinguishes, with a high degree of accuracy, between normal pregnancy, abnormal pregnancy, and non‐pregnancy‐producing embryos is reported. The variables included are (1) follicular fluid proteins alpha1‐antitrypsin, complement C3, immunoglobulin IgG2, and total protein, and total proteoglycan level separated by isoelectric focusing; (2) follicular volume; and (3) an embryo appearance rating. The study group consisted of (1) follicles which produced embryos of known performance after transfer (a) when the number of embryos transferred = the number of implantations and, (b) where one embryo transferred = no pregnancy; (2) follicles which produced oocytes which did not cleave after insemination; and (3) follicles from which no oocyte was aspirated. Canonical discriminant analysis of follicular fluid variables and follicular volume has been used to characterize the oocyte performance groups. Correct classification was achieved in 69% of normal pregnancy, 70% of abnormal pregnancy, 33% of no pregnancy, and 47% of no cleavage oocytes. An embryo appearance rating was included with the above variables for a separate discriminant analysis of only those oocytes which had formed embryos after insemination. Correct classification was achieved in 81% of normal‐pregnancy, 70% of abnormal‐pregnancy, and 70% of nopregnancy embryos.
AB - The aim of this study has been the development of a noninvasive method of predicting the pregnancy potential of human oocytes and embryos intended for in vitro fertilization and embryo replacement. A multifactorial system which distinguishes, with a high degree of accuracy, between normal pregnancy, abnormal pregnancy, and non‐pregnancy‐producing embryos is reported. The variables included are (1) follicular fluid proteins alpha1‐antitrypsin, complement C3, immunoglobulin IgG2, and total protein, and total proteoglycan level separated by isoelectric focusing; (2) follicular volume; and (3) an embryo appearance rating. The study group consisted of (1) follicles which produced embryos of known performance after transfer (a) when the number of embryos transferred = the number of implantations and, (b) where one embryo transferred = no pregnancy; (2) follicles which produced oocytes which did not cleave after insemination; and (3) follicles from which no oocyte was aspirated. Canonical discriminant analysis of follicular fluid variables and follicular volume has been used to characterize the oocyte performance groups. Correct classification was achieved in 69% of normal pregnancy, 70% of abnormal pregnancy, 33% of no pregnancy, and 47% of no cleavage oocytes. An embryo appearance rating was included with the above variables for a separate discriminant analysis of only those oocytes which had formed embryos after insemination. Correct classification was achieved in 81% of normal‐pregnancy, 70% of abnormal‐pregnancy, and 70% of nopregnancy embryos.
KW - alpha‐antitrypsin
KW - follicular fluid
KW - follicular volume
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U2 - 10.1002/mrd.1120180106
DO - 10.1002/mrd.1120180106
M3 - Article
C2 - 3509850
AN - SCOPUS:0023417633
SN - 0148-7280
VL - 18
SP - 37
EP - 55
JO - Gamete Research
JF - Gamete Research
IS - 1
ER -