Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites

Ashok J. Chavan, Hyuntae Kim, Boyd E. Haley, Stewart K. Richardson, David S. Watt

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A photoactive C-7 derivative of forskolin, [125I]-7-forskolinyl 2-(3-azido-5-hydroxy-4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the β-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labeling consistent with the presence of a forskolin binding site or sites on tubulin.

Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalBioconjugate Chemistry
Issue number4
StatePublished - Jul 1 1993

Bibliographical note

Funding Information:
We thank the National Institutes of Health (HL 20780) for their generous financial support.

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry


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