Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites

Ashok J. Chavan; Hyuntae Kim; Boyd E. Haley; Stewart K. Richardson; David S. Watt

doi:10.1021/bc00022a004

Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites

Ashok J. Chavan, Hyuntae Kim, Boyd E. Haley, Stewart K. Richardson, David S. Watt

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A photoactive C-7 derivative of forskolin, [¹²⁵I]-7-forskolinyl 2-(3-azido-5-hydroxy-4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the β-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labeling consistent with the presence of a forskolin binding site or sites on tubulin.

Original language	English
Pages (from-to)	268-274
Number of pages	7
Journal	Bioconjugate Chemistry
Volume	4
Issue number	4
DOIs	https://doi.org/10.1021/bc00022a004
State	Published - Jul 1 1993

Bibliographical note

Funding Information:
We thank the National Institutes of Health (HL 20780) for their generous financial support.

Funding

We thank the National Institutes of Health (HL 20780) for their generous financial support.

Funders	Funder number
National Institutes of Health (NIH)	HL 20780

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc00022a004

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title = "Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites",

abstract = "A photoactive C-7 derivative of forskolin, [125I]-7-forskolinyl 2-(3-azido-5-hydroxy-4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the β-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labeling consistent with the presence of a forskolin binding site or sites on tubulin.",

author = "Chavan, \{Ashok J.\} and Hyuntae Kim and Haley, \{Boyd E.\} and Richardson, \{Stewart K.\} and Watt, \{David S.\}",

note = "Funding Information: We thank the National Institutes of Health (HL 20780) for their generous financial support.",

year = "1993",

month = jul,

day = "1",

doi = "10.1021/bc00022a004",

language = "English",

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T1 - Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites

AU - Chavan, Ashok J.

AU - Kim, Hyuntae

AU - Haley, Boyd E.

AU - Richardson, Stewart K.

AU - Watt, David S.

N1 - Funding Information: We thank the National Institutes of Health (HL 20780) for their generous financial support.

PY - 1993/7/1

Y1 - 1993/7/1

N2 - A photoactive C-7 derivative of forskolin, [125I]-7-forskolinyl 2-(3-azido-5-hydroxy-4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the β-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labeling consistent with the presence of a forskolin binding site or sites on tubulin.

AB - A photoactive C-7 derivative of forskolin, [125I]-7-forskolinyl 2-(3-azido-5-hydroxy-4-iodophenoxy)acetate, enhanced GTP-promoted tubulin polymerization, preferentially bound the β-subunit, exhibited competition with the noniodinated version of the probe, and displayed saturation of labeling consistent with the presence of a forskolin binding site or sites on tubulin.

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U2 - 10.1021/bc00022a004

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Forskolin Photoaffinity Probes for the Evaluation of Tubulin Binding Sites

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

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