Fractionation and evaluation of radical scavenging peptides from in vitro digests of buckwheat protein

Yuanyuan Ma; Youling L. Xiong; Jianjun Zhai; Haining Zhu; Thomas Dziubla

doi:10.1016/j.foodchem.2009.05.024

Fractionation and evaluation of radical scavenging peptides from in vitro digests of buckwheat protein

Yuanyuan Ma, Youling L. Xiong, Jianjun Zhai, Haining Zhu, Thomas Dziubla

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2-h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2′-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS^{+{radical dot}}) and hydroxyl (^{{radical dot}}OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS^{+{radical dot}} scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralising ^{{radical dot}}OH and was 86% and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominent peptides/amino acid in these fractions.

Original language	English
Pages (from-to)	582-588
Number of pages	7
Journal	Food Chemistry
Volume	118
Issue number	3
DOIs	https://doi.org/10.1016/j.foodchem.2009.05.024
State	Published - Feb 1 2010

Bibliographical note

Funding Information:
This research was supported by a CSREES/USDA NRI grant to Y.L.X. (2008-35503-18790). The Proteomics Core directed by H.Z. is in part supported by the NIH/NCRR Center of Biomedical Research Excellence in the Molecular Basis of Human Disease (P20-RR020171) and NIH/NIEHS Superfund Basic Research Program (P42-ES007380). The NIH Shared Instrumentation Grant S10RR023684 (to H.Z.) is acknowledged.

Keywords

Buckwheat protein
Free radical scavenging
Gel filtration
In vitro digestion
Tandem mass spectrometry

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2009.05.024

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title = "Fractionation and evaluation of radical scavenging peptides from in vitro digests of buckwheat protein",

abstract = "Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2-h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2′-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS+{radical dot}) and hydroxyl ({radical dot}OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS+{radical dot} scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralising {radical dot}OH and was 86% and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominent peptides/amino acid in these fractions.",

keywords = "Buckwheat protein, Free radical scavenging, Gel filtration, In vitro digestion, Tandem mass spectrometry",

author = "Yuanyuan Ma and Xiong, {Youling L.} and Jianjun Zhai and Haining Zhu and Thomas Dziubla",

note = "Funding Information: This research was supported by a CSREES/USDA NRI grant to Y.L.X. (2008-35503-18790). The Proteomics Core directed by H.Z. is in part supported by the NIH/NCRR Center of Biomedical Research Excellence in the Molecular Basis of Human Disease (P20-RR020171) and NIH/NIEHS Superfund Basic Research Program (P42-ES007380). The NIH Shared Instrumentation Grant S10RR023684 (to H.Z.) is acknowledged. ",

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AU - Ma, Yuanyuan

AU - Xiong, Youling L.

AU - Zhai, Jianjun

AU - Zhu, Haining

AU - Dziubla, Thomas

N1 - Funding Information: This research was supported by a CSREES/USDA NRI grant to Y.L.X. (2008-35503-18790). The Proteomics Core directed by H.Z. is in part supported by the NIH/NCRR Center of Biomedical Research Excellence in the Molecular Basis of Human Disease (P20-RR020171) and NIH/NIEHS Superfund Basic Research Program (P42-ES007380). The NIH Shared Instrumentation Grant S10RR023684 (to H.Z.) is acknowledged.

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N2 - Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2-h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2′-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS+{radical dot}) and hydroxyl ({radical dot}OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS+{radical dot} scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralising {radical dot}OH and was 86% and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominent peptides/amino acid in these fractions.

AB - Buckwheat protein (BWP) isolate was subjected to a two-stage in vitro digestion (1 h pepsin followed by 2-h pancreatin at 37 °C). The antioxidant potential of the BWP digests was compared by assessing their capacity to scavenge 2,2′-azinobis (3-ethylbenzothiszoline-6-sulphonic acid) (ABTS+{radical dot}) and hydroxyl ({radical dot}OH) radicals. The 2-h pancreatin digest, which demonstrated the strongest activity against both radicals, was subjected to Sephadex G-25 gel filtration. Of the six fractions collected, fractions IV (456 Da) and VI (362 Da) showed the highest ABTS+{radical dot} scavenging activity and were 23-27% superior to mixed BWP digest (P < 0.05). Fraction VI was most effective in neutralising {radical dot}OH and was 86% and 24% more efficient (P < 0.05) than mixed BWP digest and fraction IV, respectively. LC-MS/MS identified Trp-Pro-Leu, Val-Pro-Trp, and Val-Phe-Pro-Trp (IV), Pro-Trp (V) and tryptophan (VI) to be the prominent peptides/amino acid in these fractions.

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Fractionation and evaluation of radical scavenging peptides from in vitro digests of buckwheat protein

Abstract

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Keywords

ASJC Scopus subject areas

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