Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil

S. P. Silva; L. N.P.P. Silva; E. D.L. Rodrigues; J. F. Cardoso; F. N. Tavares; W. M. Souza; C. M.P. Santos; F. M.S. Martins; I. S. Jesus; T. C. Brito; T. P.C. Moura; M. R.T. Nunes; L. M.N. Casseb; E. Silva Filho; A. R. Casseb

doi:10.4238/gmr16039719

Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil

S. P. Silva
, L. N.P.P. Silva
, E. D.L. Rodrigues
, J. F. Cardoso
, F. N. Tavares
, W. M. Souza
, C. M.P. Santos
, F. M.S. Martins
, I. S. Jesus
, T. C. Brito
, T. P.C. Moura
, M. R.T. Nunes
, L. M.N. Casseb
, E. Silva Filho
, A. R. Casseb

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.

Original language	English
Article number	gmr16039719
Journal	Genetics and Molecular Research
Volume	16
Issue number	3
DOIs	https://doi.org/10.4238/gmr16039719
State	Published - Sep 21 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors.

Funding

We are grateful for the economic support by PIBIC/IEC/CNPq/2015-2016 and Fundação de Amparo à Pesquisa do Estado de São Paulo, Brasil (Scholarships #12/24150-9 and #15/05778-5).

Funders	Funder number
PIBIC/IEC/CNPq	/2015-2016
Fundação de Amparo à Pesquisa do Estado de São Paulo	12/24150-9, 15/05778-5

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CPV-2
DNA virus
Dog
PCR
Phylogenetic analysis

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.4238/gmr16039719

Cite this

Silva, S. P., Silva, L. N. P. P., Rodrigues, E. D. L., Cardoso, J. F., Tavares, F. N., Souza, W. M., Santos, C. M. P., Martins, F. M. S., Jesus, I. S., Brito, T. C., Moura, T. P. C., Nunes, M. R. T., Casseb, L. M. N., Silva Filho, E., & Casseb, A. R. (2017). Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil. Genetics and Molecular Research, 16(3), Article gmr16039719. https://doi.org/10.4238/gmr16039719

@article{ebf20dac22674ea6ae72265730c40f50,

title = "Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil",

abstract = "With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Bel{\'e}m city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Bel{\'e}m. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Bel{\'e}m. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Bel{\'e}m CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Bel{\'e}m originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.",

keywords = "CPV-2, DNA virus, Dog, PCR, Phylogenetic analysis",

author = "Silva, \{S. P.\} and Silva, \{L. N.P.P.\} and Rodrigues, \{E. D.L.\} and Cardoso, \{J. F.\} and Tavares, \{F. N.\} and Souza, \{W. M.\} and Santos, \{C. M.P.\} and Martins, \{F. M.S.\} and Jesus, \{I. S.\} and Brito, \{T. C.\} and Moura, \{T. P.C.\} and Nunes, \{M. R.T.\} and Casseb, \{L. M.N.\} and \{Silva Filho\}, E. and Casseb, \{A. R.\}",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors.",

year = "2017",

month = sep,

day = "21",

doi = "10.4238/gmr16039719",

language = "English",

volume = "16",

journal = "Genetics and Molecular Research",

issn = "1676-5680",

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Silva, SP, Silva, LNPP, Rodrigues, EDL, Cardoso, JF, Tavares, FN, Souza, WM, Santos, CMP, Martins, FMS, Jesus, IS, Brito, TC, Moura, TPC, Nunes, MRT, Casseb, LMN, Silva Filho, E & Casseb, AR 2017, 'Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil', Genetics and Molecular Research, vol. 16, no. 3, gmr16039719. https://doi.org/10.4238/gmr16039719

TY - JOUR

T1 - Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil

AU - Silva, S. P.

AU - Silva, L. N.P.P.

AU - Rodrigues, E. D.L.

AU - Cardoso, J. F.

AU - Tavares, F. N.

AU - Souza, W. M.

AU - Santos, C. M.P.

AU - Martins, F. M.S.

AU - Jesus, I. S.

AU - Brito, T. C.

AU - Moura, T. P.C.

AU - Nunes, M. R.T.

AU - Casseb, L. M.N.

AU - Silva Filho, E.

AU - Casseb, A. R.

PY - 2017/9/21

Y1 - 2017/9/21

N2 - With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.

AB - With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing of the complete DNA sequences (NS1, NS2, VP1, and VP2 genes), the CPV-2 strain was identified as CPV-2b (Asn426Asp) circulating in Belém. The CPV-2b strain with a different change at the position Tyr324Leu was detected in all samples assessed and thus reported for the first time for the scientific community. Phylogenetic analysis indicated that Belém CPV-2b and CPV-2a strains would be related to a cluster with samples after the 1990s, suggesting that CPV-2b in Belém originated from CPV-2a circulating in Brazil after the 1990s. Potential recombination events were analyzed using RDP4 and SplitsTree4; therefore, results suggest that CPV-2 sequences here described were not potentially recombination events. Continuous monitoring and molecular characterization of CPV-2 samples are needed not only to identify possible genetic and antigenic changes that may interfere with the effectiveness of vaccines but also to bring a better understanding of the mechanisms that drive the evolution of CPV-2 in Brazil.

KW - CPV-2

KW - DNA virus

KW - Dog

KW - PCR

KW - Phylogenetic analysis

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JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

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ER -

Full-length genomic and molecular characterization of Canine parvovirus in dogs from north of Brazil

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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