Function of lanGT3, a glycosyltransferase gene involved in landomycin A biosynthesis

Andriy Luzhetskyy; Tao Liu; Marta Fedoryshyn; Bohdan Ostash; Viktor Fedorenko; Jürgen Rohr; Andreas Bechthold

doi:10.1002/cbic.200400123

Function of lanGT3, a glycosyltransferase gene involved in landomycin A biosynthesis

Andriy Luzhetskyy, Tao Liu, Marta Fedoryshyn, Bohdan Ostash, Viktor Fedorenko, Jürgen Rohr, Andreas Bechthold

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

The glycosyltransferase gene lanGT3, involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGTS in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.

Original language	English
Pages (from-to)	1567-1570
Number of pages	4
Journal	ChemBioChem
Volume	5
Issue number	11
DOIs	https://doi.org/10.1002/cbic.200400123
State	Published - Nov 5 2004

Keywords

Biosynthesis
Glycosylation
Glycosyltransferases
Landomycin
Olivose

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200400123

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abstract = "The glycosyltransferase gene lanGT3, involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGTS in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.",

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AU - Liu, Tao

AU - Fedoryshyn, Marta

AU - Ostash, Bohdan

AU - Fedorenko, Viktor

AU - Rohr, Jürgen

AU - Bechthold, Andreas

PY - 2004/11/5

Y1 - 2004/11/5

N2 - The glycosyltransferase gene lanGT3, involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGTS in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.

AB - The glycosyltransferase gene lanGT3, involved in the biosynthesis of the angucyclic antibiotic landomycin A, has been characterised by targeted gene deletion. A lanGT3 mutant was shown to produce landomycin E, which consists of a trisaccharide side chain attached to the polyketide moiety. Expression of lanGTS in the mutant restored landomycin A production. Our results indicate that LanGT3 is responsible for the transfer of the fourth sugar during landomycin A biosynthesis.

KW - Biosynthesis

KW - Glycosylation

KW - Glycosyltransferases

KW - Landomycin

KW - Olivose

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Function of lanGT3, a glycosyltransferase gene involved in landomycin A biosynthesis

Abstract

Keywords

ASJC Scopus subject areas

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