Functional analysis of the Rubisco large subunit (ε)N-methyltransferase promoter from tobacco and its regulation by light in soybean hairy roots

M. Mazarei, Z. Ying, R. L. Houtz

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) (ε)N-methyltransferase (Rubisco LSMT) catalyzes post-translational methylation of the ε-amino group of lysine-14 in the LS of Rubisco. The entire nucleotide sequence for the tobacco (Nicotiana tabacum) Rubisco LSMT (rbcMT-T) gene including the putative promoter region was recently reported, and sequence analysis of the promoter region revealed seven GT-1 motifs. The ability of several truncated rbcMT-T promoter fragments to confer light responsiveness to reporter gene expression in transgenic soybean (Glycine max) hairy roots was examined. Chimeric constructs consisting of the rbcMT-T promoter fused to a bacterial β-glucuronidase (GUS) reporter gene and transferred to soybean via Agrobacterium rhizogenes were evaluated. The rbcMT-T promoter fragments conferred expression of the reporter gene in transgenic hairy roots, callus, and cell suspension cultures based on histochemical and fluorometric GUS analyses. The results suggest a quantitative role for the number of GT-1 motifs in determining differential expression between light and dark conditions.

Original languageEnglish
Pages (from-to)907-912
Number of pages6
JournalPlant Cell Reports
Volume17
Issue number12
DOIs
StatePublished - 1998

Bibliographical note

Funding Information:
Acknowledgements We would like to thank Dr. Stephen K. Farrand for supplying the A. rhizogenes strain, and Malcolm Royer for excellent technical assistance. This work was supported by the United States Department of Energy Grant DE-F05-92ER20075 to R. L. H., and is published as Kentucky Agricultural Experiment Station Report no. 98-11-41.

Keywords

  • (ε)N-methyltransferase
  • Agrobacterium rhizogenes
  • GT-1
  • Promoter
  • Rubisco

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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