Functional characterization of the promoter for the mouse SPTLC2 gene, which encodes subunit 2 of serine palmitoyltransferase

Stephen C. Linn; Lindsay M. Andras; Hee Sook Kim; Jia Wei; M. Marek Nagiec; Robert C. Dickson; Alfred H. Merrill

doi:10.1016/j.febslet.2006.10.025

Functional characterization of the promoter for the mouse SPTLC2 gene, which encodes subunit 2 of serine palmitoyltransferase

Stephen C. Linn, Lindsay M. Andras, Hee Sook Kim, Jia Wei, M. Marek Nagiec, Robert C. Dickson, Alfred H. Merrill

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A series of luciferase reporter constructs was prepared from a 1035-bp fragment of mouse genomic DNA flanking the 5′-coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full-length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site-directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2.

Original language	English
Pages (from-to)	6217-6223
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	26
DOIs	https://doi.org/10.1016/j.febslet.2006.10.025
State	Published - Nov 13 2006

Bibliographical note

Funding Information:
This work was supported by NIH Grants GM46368 and GM069338 (Lipid MAPS Consortium) (to A.H.M.) and GM41302 (to R.C.D.).

Funding

This work was supported by NIH Grants GM46368 and GM069338 (Lipid MAPS Consortium) (to A.H.M.) and GM41302 (to R.C.D.).

Funders	Funder number
National Institutes of Health (NIH)	GM41302, GM069338
National Institute of General Medical Sciences	R01GM046368

Keywords

Luciferase reporter construct
Serine palmitoyltransferase
Sphingolipid biosynthesis
Transcription

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.10.025

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title = "Functional characterization of the promoter for the mouse SPTLC2 gene, which encodes subunit 2 of serine palmitoyltransferase",

abstract = "A series of luciferase reporter constructs was prepared from a 1035-bp fragment of mouse genomic DNA flanking the 5′-coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full-length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site-directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2.",

keywords = "Luciferase reporter construct, Serine palmitoyltransferase, Sphingolipid biosynthesis, Transcription",

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AU - Linn, Stephen C.

AU - Andras, Lindsay M.

AU - Kim, Hee Sook

AU - Wei, Jia

AU - Nagiec, M. Marek

AU - Dickson, Robert C.

AU - Merrill, Alfred H.

N1 - Funding Information: This work was supported by NIH Grants GM46368 and GM069338 (Lipid MAPS Consortium) (to A.H.M.) and GM41302 (to R.C.D.).

PY - 2006/11/13

Y1 - 2006/11/13

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AB - A series of luciferase reporter constructs was prepared from a 1035-bp fragment of mouse genomic DNA flanking the 5′-coding sequence for the SPTLC2 subunit of serine palmitoyltransferase, the initial enzyme of de novo sphingolipid biosynthesis. The full-length DNA fragment promoted strong reporter gene expression in NIH3T3 cells while deletion and site-directed mutagenesis indicated that the proximal 335 bp contain initiator and downstream promoter elements, two proximal GC boxes that appear to stimulate transcription in a cooperative manner, and several additional elements whose activity cannot be accounted for by known factor binding sites. These findings provide insight into the control mechanisms for transcription of mammalian SPTLC2.

KW - Luciferase reporter construct

KW - Serine palmitoyltransferase

KW - Sphingolipid biosynthesis

KW - Transcription

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Functional characterization of the promoter for the mouse SPTLC2 gene, which encodes subunit 2 of serine palmitoyltransferase

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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