Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast

Sabire Ozcan; Amber L. Mosley; Bishwa K. Aryal

doi:10.1016/S0006-291X(02)00747-7

Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast

Sabire Ozcan
, Amber L. Mosley
, Bishwa K. Aryal

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The pancreas-specific transcription factor Pdx-1 is important for pancreas development and β-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type II diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast.

Original language	English
Pages (from-to)	724-729
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	295
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(02)00747-7
State	Published - 2002

Bibliographical note

Funding Information:
This work was supported by a Juvenile Diabetes Foundation Career Development Award (to S.O.) We thank Alan Permutt (Washington University, St. Louis) for providing the cDNA for the mouse PDX-1 gene, Michael German (UCSD) for the plasmid containing the A3 region, and Fang Fang for excellent technical assistance.

Funding

This work was supported by a Juvenile Diabetes Foundation Career Development Award (to S.O.) We thank Alan Permutt (Washington University, St. Louis) for providing the cDNA for the mouse PDX-1 gene, Michael German (UCSD) for the plasmid containing the A3 region, and Fang Fang for excellent technical assistance.

Funders
University of California San Diego Health
Juvenile Diabetes Research Foundation United States of America

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Activator
Gene expression
Pancreas
Pdx-1
Transcription factor
Yeast

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(02)00747-7

Cite this

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title = "Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast",

abstract = "The pancreas-specific transcription factor Pdx-1 is important for pancreas development and β-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type II diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast.",

keywords = "Activator, Gene expression, Pancreas, Pdx-1, Transcription factor, Yeast",

author = "Sabire Ozcan and Mosley, \{Amber L.\} and Aryal, \{Bishwa K.\}",

note = "Funding Information: This work was supported by a Juvenile Diabetes Foundation Career Development Award (to S.O.) We thank Alan Permutt (Washington University, St. Louis) for providing the cDNA for the mouse PDX-1 gene, Michael German (UCSD) for the plasmid containing the A3 region, and Fang Fang for excellent technical assistance.",

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AU - Ozcan, Sabire

AU - Mosley, Amber L.

AU - Aryal, Bishwa K.

N1 - Funding Information: This work was supported by a Juvenile Diabetes Foundation Career Development Award (to S.O.) We thank Alan Permutt (Washington University, St. Louis) for providing the cDNA for the mouse PDX-1 gene, Michael German (UCSD) for the plasmid containing the A3 region, and Fang Fang for excellent technical assistance.

PY - 2002

Y1 - 2002

N2 - The pancreas-specific transcription factor Pdx-1 is important for pancreas development and β-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type II diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast.

AB - The pancreas-specific transcription factor Pdx-1 is important for pancreas development and β-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type II diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast.

KW - Activator

KW - Gene expression

KW - Pancreas

KW - Pdx-1

KW - Transcription factor

KW - Yeast

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Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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