Abstract
We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.
Original language | English |
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Pages (from-to) | 195-200 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 275 |
Issue number | 1-2 |
DOIs | |
State | Published - Nov 26 1990 |
Keywords
- Na channel
- Na current
- Saxitoxin
- Tetrodotoxin
- Xenopus oocyte
- cDNA expression
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology