Functional expression of the rat heart I Na+ channel isoform Demonstration of properties characteristic of native cardiac Na+ channels

Leanne L. Cribbs; Jonathan Satin; Harry A. Fozzard; Richard B. Rogart

doi:10.1016/0014-5793(90)81470-9

Functional expression of the rat heart I Na⁺ channel isoform Demonstration of properties characteristic of native cardiac Na⁺ channels

Leanne L. Cribbs, Jonathan Satin, Harry A. Fozzard, Richard B. Rogart

Physiology

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We describe the expression of functional Na⁺ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na⁺ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na⁺ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.

Original language	English
Pages (from-to)	195-200
Number of pages	6
Journal	FEBS Letters
Volume	275
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(90)81470-9
State	Published - Nov 26 1990

Keywords

Na channel
Na current
Saxitoxin
Tetrodotoxin
Xenopus oocyte
cDNA expression

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(90)81470-9

Cite this

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title = "Functional expression of the rat heart I Na+ channel isoform Demonstration of properties characteristic of native cardiac Na+ channels",

abstract = "We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.",

keywords = "Na channel, Na current, Saxitoxin, Tetrodotoxin, Xenopus oocyte, cDNA expression",

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AU - Cribbs, Leanne L.

AU - Satin, Jonathan

AU - Fozzard, Harry A.

AU - Rogart, Richard B.

PY - 1990/11/26

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N2 - We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.

AB - We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.

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KW - Na current

KW - Saxitoxin

KW - Tetrodotoxin

KW - Xenopus oocyte

KW - cDNA expression

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