Functional expression of the rat heart I Na+ channel isoform Demonstration of properties characteristic of native cardiac Na+ channels

Leanne L. Cribbs, Jonathan Satin, Harry A. Fozzard, Richard B. Rogart

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalFEBS Letters
Volume275
Issue number1-2
DOIs
StatePublished - Nov 26 1990

Keywords

  • Na channel
  • Na current
  • Saxitoxin
  • Tetrodotoxin
  • Xenopus oocyte
  • cDNA expression

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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