Gene expression dynamics during diabetic periodontitis

O. M. Andriankaja, J. Galicia, G. Dong, W. Xiao, F. Alawi, D. T. Graves

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Diabetes impairs the resolution of periodontal inflammation. We explored pathways altered by inflammation in the diabetic periodontium by using ligatures to induce periodontitis in type-2 diabetic Goto-Kakizaki rats. Ligatures were removed after 7 days, and rats were then treated with TNF inhibitor (pegsunercept) or vehicle alone and euthanized 4 days later. RNA was extracted from periodontal tissue, examined by mRNA profiling, and further analyzed by functional criteria. We found that 1,754 genes were significantly up-regulated and 1,243 were down-regulated by pegsunercept (p < 0.05). Functional analysis revealed up-regulation of neuron-associated and retina-associated gene clusters as well as those related to cell activity and signaling. Others were down-regulated by TNF inhibition and included genes associated with host defense, apoptosis, cell signaling and activity, and coagulation/hemostasis/ complement. For selected genes, findings with microarray and rt-PCR agreed. PPAR-α was investigated further by immunohistochemistry due to its anti-inflammatory function and was found to be up-regulated in the gingiva during the resolution of periodontal inflammation and suppressed by diabetes. The results indicate that diabetes-enhanced inflammation both up- and down-regulates genes involved in cellular activity and cell signaling, while it predominantly up-regulates genes involved in the host response, apoptosis, and coagulation/homeostasis/complement and down-regulates mRNA levels of neuron, retina, and energy/metabolism-associated genes.

Original languageEnglish
Pages (from-to)1160-1165
Number of pages6
JournalJournal of Dental Research
Issue number12
StatePublished - Dec 2012

Bibliographical note

Funding Information:
This study was supported by NIDCR grant DE017732 and a Research Supplement to Promote Diversity.


  • GSEA
  • diabetes
  • inflammation
  • microarray
  • periodontal disease(s)

ASJC Scopus subject areas

  • Dentistry (all)


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