Gene flow in populations of the seven-spotted lady beetle, Coccinella septempunctata

E. S. Krafsur, J. J. Obrycki, R. V. Flanders

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Polyacrylamide gel electrophoresis was used to reveal gene diversity in exotic North American Coccinella Septempunctata populations. This lady beetle recently spread across the northern Nearctic. Sixteen of 28 putative loci were polymorphic; average gene diversity for all loci was 0.1598 ± 0.041. Gene frequencies were estimated at eight polymorphic loci in natural North American beetles from Arkansas, Delaware, IA, Kansas, New York, Oregon, and Michigan. Also studied were F2 beetles from four laboratory colonies that originated in Eurasia, along with field-collected beetles from France, Greece, and Sicily. Gene diversity among the Nearctic beetles was as great as that among the Palearctic beetles. Analysis of variance by Wright's method showed that only 29% of the variance in gene frequencies was between USDA cultures, Palearctic, and Nearctic beetles; 71% of the genetic variance was shared by beetles within the 21 subpopulations. No evidence of bottlenecks of drift was detected among the Nearctic subpopulations, and gene flow was essentially unrestricted.

Original languageEnglish
Pages (from-to)440-444
Number of pages5
JournalJournal of Heredity
Volume83
Issue number6
DOIs
StatePublished - 1992

Bibliographical note

Funding Information:
From the Department of Entomology, Iowa State University, Ames, IA 50011 (Krafsur and Obrycki) and the National Biological Control Laboratory, USDA-APH1S-PPQ, Niles, Michigan (Flanders). Journal paper no. J-14809 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Project no. 2949. Thanks to T. J. Kring and J. R. Ruberson, University of Arkansas; J. R. Nechols, Kansas State University; B. Gollands, Cornell University; S. Gage, Michigan State University; and P. Schaefer, USDA-ARS for supplying beetles. This research was partly supported by USDA-APHIS Co-operative Research Agreement no. 12-34-81-0168-GR. The subpopulation gene frequencies are available from the senior author on request ([email protected]).

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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