Gene flow in populations of the seven-spotted lady beetle, Coccinella septempunctata

E. S. Krafsur; J. J. Obrycki; R. V. Flanders

doi:10.1093/oxfordjournals.jhered.a111248

Gene flow in populations of the seven-spotted lady beetle, Coccinella septempunctata

E. S. Krafsur, J. J. Obrycki, R. V. Flanders

Entomology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Polyacrylamide gel electrophoresis was used to reveal gene diversity in exotic North American Coccinella Septempunctata populations. This lady beetle recently spread across the northern Nearctic. Sixteen of 28 putative loci were polymorphic; average gene diversity for all loci was 0.1598 ± 0.041. Gene frequencies were estimated at eight polymorphic loci in natural North American beetles from Arkansas, Delaware, IA, Kansas, New York, Oregon, and Michigan. Also studied were F₂ beetles from four laboratory colonies that originated in Eurasia, along with field-collected beetles from France, Greece, and Sicily. Gene diversity among the Nearctic beetles was as great as that among the Palearctic beetles. Analysis of variance by Wright's method showed that only 29% of the variance in gene frequencies was between USDA cultures, Palearctic, and Nearctic beetles; 71% of the genetic variance was shared by beetles within the 21 subpopulations. No evidence of bottlenecks of drift was detected among the Nearctic subpopulations, and gene flow was essentially unrestricted.

Original language	English
Pages (from-to)	440-444
Number of pages	5
Journal	Journal of Heredity
Volume	83
Issue number	6
DOIs	https://doi.org/10.1093/oxfordjournals.jhered.a111248
State	Published - 1992

Bibliographical note

Funding Information:
From the Department of Entomology, Iowa State University, Ames, IA 50011 (Krafsur and Obrycki) and the National Biological Control Laboratory, USDA-APH1S-PPQ, Niles, Michigan (Flanders). Journal paper no. J-14809 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Project no. 2949. Thanks to T. J. Kring and J. R. Ruberson, University of Arkansas; J. R. Nechols, Kansas State University; B. Gollands, Cornell University; S. Gage, Michigan State University; and P. Schaefer, USDA-ARS for supplying beetles. This research was partly supported by USDA-APHIS Co-operative Research Agreement no. 12-34-81-0168-GR. The subpopulation gene frequencies are available from the senior author on request ([email protected]).

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Genetics
Genetics(clinical)

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10.1093/oxfordjournals.jhered.a111248

Cite this

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title = "Gene flow in populations of the seven-spotted lady beetle, Coccinella septempunctata",

abstract = "Polyacrylamide gel electrophoresis was used to reveal gene diversity in exotic North American Coccinella Septempunctata populations. This lady beetle recently spread across the northern Nearctic. Sixteen of 28 putative loci were polymorphic; average gene diversity for all loci was 0.1598 ± 0.041. Gene frequencies were estimated at eight polymorphic loci in natural North American beetles from Arkansas, Delaware, IA, Kansas, New York, Oregon, and Michigan. Also studied were F2 beetles from four laboratory colonies that originated in Eurasia, along with field-collected beetles from France, Greece, and Sicily. Gene diversity among the Nearctic beetles was as great as that among the Palearctic beetles. Analysis of variance by Wright's method showed that only 29% of the variance in gene frequencies was between USDA cultures, Palearctic, and Nearctic beetles; 71% of the genetic variance was shared by beetles within the 21 subpopulations. No evidence of bottlenecks of drift was detected among the Nearctic subpopulations, and gene flow was essentially unrestricted.",

author = "Krafsur, {E. S.} and Obrycki, {J. J.} and Flanders, {R. V.}",

note = "Funding Information: From the Department of Entomology, Iowa State University, Ames, IA 50011 (Krafsur and Obrycki) and the National Biological Control Laboratory, USDA-APH1S-PPQ, Niles, Michigan (Flanders). Journal paper no. J-14809 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Project no. 2949. Thanks to T. J. Kring and J. R. Ruberson, University of Arkansas; J. R. Nechols, Kansas State University; B. Gollands, Cornell University; S. Gage, Michigan State University; and P. Schaefer, USDA-ARS for supplying beetles. This research was partly supported by USDA-APHIS Co-operative Research Agreement no. 12-34-81-0168-GR. The subpopulation gene frequencies are available from the senior author on request ([email protected]).",

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N1 - Funding Information: From the Department of Entomology, Iowa State University, Ames, IA 50011 (Krafsur and Obrycki) and the National Biological Control Laboratory, USDA-APH1S-PPQ, Niles, Michigan (Flanders). Journal paper no. J-14809 of the Iowa Agriculture and Home Economics Experiment Station, Ames. Project no. 2949. Thanks to T. J. Kring and J. R. Ruberson, University of Arkansas; J. R. Nechols, Kansas State University; B. Gollands, Cornell University; S. Gage, Michigan State University; and P. Schaefer, USDA-ARS for supplying beetles. This research was partly supported by USDA-APHIS Co-operative Research Agreement no. 12-34-81-0168-GR. The subpopulation gene frequencies are available from the senior author on request ([email protected]).

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N2 - Polyacrylamide gel electrophoresis was used to reveal gene diversity in exotic North American Coccinella Septempunctata populations. This lady beetle recently spread across the northern Nearctic. Sixteen of 28 putative loci were polymorphic; average gene diversity for all loci was 0.1598 ± 0.041. Gene frequencies were estimated at eight polymorphic loci in natural North American beetles from Arkansas, Delaware, IA, Kansas, New York, Oregon, and Michigan. Also studied were F2 beetles from four laboratory colonies that originated in Eurasia, along with field-collected beetles from France, Greece, and Sicily. Gene diversity among the Nearctic beetles was as great as that among the Palearctic beetles. Analysis of variance by Wright's method showed that only 29% of the variance in gene frequencies was between USDA cultures, Palearctic, and Nearctic beetles; 71% of the genetic variance was shared by beetles within the 21 subpopulations. No evidence of bottlenecks of drift was detected among the Nearctic subpopulations, and gene flow was essentially unrestricted.

AB - Polyacrylamide gel electrophoresis was used to reveal gene diversity in exotic North American Coccinella Septempunctata populations. This lady beetle recently spread across the northern Nearctic. Sixteen of 28 putative loci were polymorphic; average gene diversity for all loci was 0.1598 ± 0.041. Gene frequencies were estimated at eight polymorphic loci in natural North American beetles from Arkansas, Delaware, IA, Kansas, New York, Oregon, and Michigan. Also studied were F2 beetles from four laboratory colonies that originated in Eurasia, along with field-collected beetles from France, Greece, and Sicily. Gene diversity among the Nearctic beetles was as great as that among the Palearctic beetles. Analysis of variance by Wright's method showed that only 29% of the variance in gene frequencies was between USDA cultures, Palearctic, and Nearctic beetles; 71% of the genetic variance was shared by beetles within the 21 subpopulations. No evidence of bottlenecks of drift was detected among the Nearctic subpopulations, and gene flow was essentially unrestricted.

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Gene flow in populations of the seven-spotted lady beetle, Coccinella septempunctata

Abstract

Bibliographical note

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