Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation

Maythem AL-Amery, Ashley Fowler, Jason M. Unrine, Paul Armstrong, Elizabeth Maghirang, Kai Su, Julia de Melo, Feng Jie Yuan, Qing Yao Shu, David Hildebrand

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Soybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo-inositol 1-phosphate synthase (Gm-lpa-TW75-1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm-lpa-TW75-1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm-lpa-TW75-1. Phosphate (Pi) is higher in Gm-lpa-TW75-1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm-lpa-TW75-1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.

Original languageEnglish
Pages (from-to)469-477
Number of pages9
JournalLipids
Volume55
Issue number5
DOIs
StatePublished - Sep 1 2020

Bibliographical note

Funding Information:
We would like to thank Dr. Bruce Downie (Department of Horticulture), Tonya Morgan (Center for Applied Energy Research), Shristi Shrestha, John Johnson and James Crutchfield, for their help and support. In addition, we thank the sponsor of the first author (HCED), The Committee for Higher Education and Development in IRAQ. Partial support was provided by the Kentucky Agricultural Experiment Station. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval for the exclusion of other products that may also be suitable. USDA is an equal opportunity provider and employer.

Funding Information:
We would like to thank Dr. Bruce Downie (Department of Horticulture), Tonya Morgan (Center for Applied Energy Research), Shristi Shrestha, John Johnson and James Crutchfield, for their help and support. In addition, we thank the sponsor of the first author (HCED), The Committee for Higher Education and Development in IRAQ. Partial support was provided by the Kentucky Agricultural Experiment Station. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval for the exclusion of other products that may also be suitable. USDA is an equal opportunity provider and employer.

Publisher Copyright:
© 2020 AOCS

Keywords

  • Glycine max
  • High oil
  • High phosphate
  • High protein
  • Low phytate

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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