Generation of a conditional allele of the mouse prostaglandin EP 4 receptor

André Schneider, You Fei Guan, Yahua Zhang, Mark A. Magnuson, Cathy Pettepher, Charles D. Loftin, Robert Langenbach, Richard M. Breyer, Matthew D. Breyer

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Genetic disruption of the mouse EP4 receptor results in perinatal lethality associated with persistent patent ductus areteriosus (PDA). To circumvent this, an EP4 allele amenable to conditional deletion using the Cre/loxP system was generated. The targeting construct was comprised of a floxed exon2 in tandem with the neomycin-resistance gene in intron 2, flanked by third 3′ LoxP site. Mice homozygous for the targeted allele (EP4lox+neo/lox+neo), or following its Cre-mediated deletion (EP4del/del), also die within hours of birth with PDA. In contrast, mice homozygous for a partially recombined allele, retaining exon2 but lacking neo (EP4flox/flox), are viable and show no overt phenotype. Postnatal deletion of the floxed EP4 gene is efficiently achieved in the liver and kidney in a transgenic mouse expressing the inducible Mx1Cre recombinase. The EP4flox mouse should provide a useful reagent with which to examine the physiologic roles of the EP4 receptor.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
Issue number1
StatePublished - Sep 2004


  • Dinoprostone
  • Ductus arteriosus

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology


Dive into the research topics of 'Generation of a conditional allele of the mouse prostaglandin EP 4 receptor'. Together they form a unique fingerprint.

Cite this