Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions

I. Dobrinski, J. R. Jacob, B. C. Tennant, B. A. Ball

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.

Original languageEnglish
Pages (from-to)875-885
Number of pages11
JournalTheriogenology
Volume52
Issue number5
DOIs
StatePublished - Oct 1 1999

Bibliographical note

Funding Information:
D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.

Funding

D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.

FundersFunder number
Harry M. Zweig Memorial Fund for Equine Research

    Keywords

    • Horse
    • Oviduct
    • SV40
    • Sperm binding

    ASJC Scopus subject areas

    • Small Animals
    • Food Animals
    • Animal Science and Zoology
    • Equine

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