Abstract
Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.
Original language | English |
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Pages (from-to) | 875-885 |
Number of pages | 11 |
Journal | Theriogenology |
Volume | 52 |
Issue number | 5 |
DOIs | |
State | Published - Oct 1 1999 |
Bibliographical note
Funding Information:D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.
Funding
D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.
Funders | Funder number |
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Harry M. Zweig Memorial Fund for Equine Research |
Keywords
- Horse
- Oviduct
- SV40
- Sperm binding
ASJC Scopus subject areas
- Small Animals
- Food Animals
- Animal Science and Zoology
- Equine