Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis

Philip J. Bridges; Yongbum Koo; Dong Wook Kang; Susan Hudgins-Spivey; Zi Jian Lan; Xueping Xu; Francesco DeMayo; Austin Cooney; Che Myong Ko

doi:10.1002/dvg.20428

Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis

Philip J. Bridges, Yongbum Koo, Dong Wook Kang, Susan Hudgins-Spivey, Zi Jian Lan, Xueping Xu, Francesco DeMayo, Austin Cooney, Che Myong Ko

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

A transgenic mouse line that expresses iCre under regulation of the Cytochrome P₄₅₀ 17α-hydroxylase/17, 20-lyase (Cyp17) promoter was developed as a novel transgenic mouse model for the conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional deletion of the estrogen receptor alpha (Esr1) gene in the theca/interstitial and Leydig cells of the female and male gonad, respectively. These mice will prove a powerful tool to inactivate genes in the gonad in a cell-specific manner.

Original language	English
Pages (from-to)	499-505
Number of pages	7
Journal	Genesis
Volume	46
Issue number	9
DOIs	https://doi.org/10.1002/dvg.20428
State	Published - 2008

Keywords

Cre
Estrogen receptor alpha
Leydig cells
Theca interstitial

ASJC Scopus subject areas

Genetics
Endocrinology
Cell Biology

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10.1002/dvg.20428

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title = "Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis",

abstract = "A transgenic mouse line that expresses iCre under regulation of the Cytochrome P450 17α-hydroxylase/17, 20-lyase (Cyp17) promoter was developed as a novel transgenic mouse model for the conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional deletion of the estrogen receptor alpha (Esr1) gene in the theca/interstitial and Leydig cells of the female and male gonad, respectively. These mice will prove a powerful tool to inactivate genes in the gonad in a cell-specific manner.",

keywords = "Cre, Estrogen receptor alpha, Leydig cells, Theca interstitial",

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T1 - Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis

AU - Bridges, Philip J.

AU - Koo, Yongbum

AU - Kang, Dong Wook

AU - Hudgins-Spivey, Susan

AU - Lan, Zi Jian

AU - Xu, Xueping

AU - DeMayo, Francesco

AU - Cooney, Austin

AU - Ko, Che Myong

PY - 2008

Y1 - 2008

N2 - A transgenic mouse line that expresses iCre under regulation of the Cytochrome P450 17α-hydroxylase/17, 20-lyase (Cyp17) promoter was developed as a novel transgenic mouse model for the conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional deletion of the estrogen receptor alpha (Esr1) gene in the theca/interstitial and Leydig cells of the female and male gonad, respectively. These mice will prove a powerful tool to inactivate genes in the gonad in a cell-specific manner.

AB - A transgenic mouse line that expresses iCre under regulation of the Cytochrome P450 17α-hydroxylase/17, 20-lyase (Cyp17) promoter was developed as a novel transgenic mouse model for the conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional deletion of the estrogen receptor alpha (Esr1) gene in the theca/interstitial and Leydig cells of the female and male gonad, respectively. These mice will prove a powerful tool to inactivate genes in the gonad in a cell-specific manner.

KW - Cre

KW - Estrogen receptor alpha

KW - Leydig cells

KW - Theca interstitial

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JF - Genesis

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Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis

Abstract

Keywords

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