Abstract
Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.
Original language | English |
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Pages (from-to) | 675-678 |
Number of pages | 4 |
Journal | ChemBioChem |
Volume | 6 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2005 |
Keywords
- Antitumor agents
- Biosynthesis
- Glycosylation
- Hydroxylation
- Landomycin
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry