Generation of novel landomycins M and O through targeted gene disruption

Andriy Luzhetskyy, Lili Zhu, Miranda Gibson, Marta Fedoryshyn, Clemens Dürr, Carsten Hofmann, Dirk Hoffmeister, Bohdan Ostash, Cynthia Mattingly, Val Adams, Victor Fedorenko, Jürgen Rohr, Andreas Bechthold

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
JournalChembiochem : a European journal of chemical biology
Volume6
Issue number4
DOIs
StatePublished - Apr 2005

Keywords

  • Antitumor agents
  • Biosynthesis
  • Glycosylation
  • Hydroxylation
  • Landomycin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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