Generation of novel landomycins M and O through targeted gene disruption

Andriy Luzhetskyy; Lili Zhu; Miranda Gibson; Marta Fedoryshyn; Clemens Dürr; Carsten Hofmann; Dirk Hoffmeister; Bohdan Ostash; Cynthia Mattingly; Val Adams; Victor Fedorenko; Jürgen Rohr; Andreas Bechthold

doi:10.1002/cbic.200400316

Generation of novel landomycins M and O through targeted gene disruption

Andriy Luzhetskyy
, Lili Zhu
, Miranda Gibson
, Marta Fedoryshyn
, Clemens Dürr
, Carsten Hofmann
, Dirk Hoffmeister
, Bohdan Ostash
, Cynthia Mattingly
, Val Adams
, Victor Fedorenko
, Jürgen Rohr
, Andreas Bechthold

Research output: Contribution to journal › Article › peer-review

45 Citations (SciVal)

Abstract

Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.

Original language	English
Pages (from-to)	675-678
Number of pages	4
Journal	ChemBioChem
Volume	6
Issue number	4
DOIs	https://doi.org/10.1002/cbic.200400316
State	Published - Apr 2005

Keywords

Antitumor agents
Biosynthesis
Glycosylation
Hydroxylation
Landomycin

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200400316

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title = "Generation of novel landomycins M and O through targeted gene disruption",

abstract = "Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.",

keywords = "Antitumor agents, Biosynthesis, Glycosylation, Hydroxylation, Landomycin",

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doi = "10.1002/cbic.200400316",

language = "English",

volume = "6",

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TY - JOUR

T1 - Generation of novel landomycins M and O through targeted gene disruption

AU - Luzhetskyy, Andriy

AU - Zhu, Lili

AU - Gibson, Miranda

AU - Fedoryshyn, Marta

AU - Dürr, Clemens

AU - Hofmann, Carsten

AU - Hoffmeister, Dirk

AU - Ostash, Bohdan

AU - Mattingly, Cynthia

AU - Adams, Val

AU - Fedorenko, Victor

AU - Rohr, Jürgen

AU - Bechthold, Andreas

PY - 2005/4

Y1 - 2005/4

N2 - Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.

AB - Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis, Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O.

KW - Antitumor agents

KW - Biosynthesis

KW - Glycosylation

KW - Hydroxylation

KW - Landomycin

UR - https://www.scopus.com/pages/publications/21044435505

UR - https://www.scopus.com/pages/publications/21044435505#tab=citedBy

U2 - 10.1002/cbic.200400316

DO - 10.1002/cbic.200400316

M3 - Article

C2 - 15812784

AN - SCOPUS:21044435505

SN - 1439-4227

VL - 6

SP - 675

EP - 678

JO - ChemBioChem

JF - ChemBioChem

IS - 4

ER -

Generation of novel landomycins M and O through targeted gene disruption

Abstract

Keywords

ASJC Scopus subject areas

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