Generation of reactive oxygen species by quartz particles and itsimplication for cellular damage

Xianglin Shi, Yan Mao, Lambert N. Daniel, Umberto Saffiotti, Nar S. Dalai, Val Vallyathan

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


Using wet analytical chemistry and electron spin resonance spin trapping, we have shown that aqueous suspensions of freshly fractured quartz particles generated hydrogen peroxide, hydroxyl radical, superoxide radical, and singlet oxygen. Superoxide dismutase partiallyinhibited the hydroxyl radical yield, whereas catalase suppressed it.Hydrogen peroxide enhanced the hydroxyl radical generation, whiledeferoxamine decreased it. Oxygen consumption measurementsshowed that freshly fractured quartz particles consumed molecularoxygen. Electrophoretic assays showed that freshly fractured quartzparticles induced DNA double-strand breakage, which was inhibitedby catalase. In an argon atmosphere DNA damage was suppressed, showing that molecular oxygen is required for quartz-induced DNAdamage. Quartz particles also caused dose-dependent lipid peroxidation as measured by malondialdehyde formation. Superoxide dismutase, catalase, and sodium benzoate inhibited quartz-induced lipidperoxidation by 49, 52, and 75 percent, respectively. The resultsdemonstrate that reaction of freshly fractured quartz particles withaqueous medium and hydrogen peroxide generates reactive oxygenspecies, which may play an important role in the mechanism ofquartz-induced cellular injury.

Original languageEnglish
Pages (from-to)1138-1144
Number of pages7
JournalApplied Occupational and Environmental Hygiene
Issue number12
StatePublished - Dec 1995

Bibliographical note

Funding Information:
West Virginia University's contribution was supported by NIOSH grant U60-CCU306149-01-1.

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health


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