Survival of mice during the acute stage of Toxoplasma gondii infection was not influenced by the MHC Class I gene, Ld, but was influenced by the MHC Class II genes, Ia and Ie. As unexplained variability was noted in our initial studies of influence of the Ld gene on survival, influence of the Ld gene region on survival in the presence of a number of variables was studied. Although route of administration and dose of parasites, and age and gender of the mice markedly influenced outcome of T. gondii infection, the Class I Ld gene did not modify survival in any of these circumstances. In separate studies, using mice with a differing genetic background, i.e. H-2b, C57BL/10 mice, presence of Ia or Ie alone diminished survival even though presence of Ia reduced parasite burden. When neither or both the Ia and Ie genes were present together, survival was greater. In separate analyses of our studies of A × B B × A recombinant inbred mice, similar influences of MHC genes on survival and parasite burden following peroral infection were confirmed. Previously undescribed associations of novel genetic loci and survival and parasite burden also were identified. Genetic loci associated with enhanced survival included D8Mit42, D1Mit3, Iapls1-16, D8Mit14, Hoxb, Mpmv29, Pmv45, and Emv-2; genetic loci associated with reduced parasite burden included H-2, D17Mit62, D17Mit83, D17Mit21, D17Mit34, D17Mit47, D18Mit4, and Gln3-5. These studies demonstrate the importance of MHC region genes (but not Ld) for survival, and the influence of other novel genes, and endogenous and exogenous variables on survival and parasite burden specified by host genes following T. gondii infection.
|Number of pages||7|
|Journal||International Journal for Parasitology|
|State||Published - 2002|
Bibliographical noteFunding Information:
We thank V. Aitchison, I. Buscher, and K. Zawisza for their assistance in preparation of the manuscript. This work was supported by the NIH NIAID TMP program (R01 AI 16945), and the Research to Prevent Blindness Foundation. D.M. is a recipient of a Career Development Award from the Research to Prevent Blindness Foundation; R.McL. is the Jules and Doris Stein RPB Professor at the University of Chicago.
- L gene
- MHC gene
- Recombinant inbred mouse
- Toxoplasma gondii
ASJC Scopus subject areas
- Infectious Diseases