Genetic and antigenic characteristics of a human influenza C virus clinical isolate

Runxia Liu, Zizhang Sheng, Tao Lin, Chithra Sreenivasan, Rongruan Gao, Milton Thomas, Julian Druce, Ben M. Hause, Radhey S. Kaushik, Feng Li, Dan Wang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Unlike influenza A and B viruses that infect humans and cause severe diseases in seasonal epidemics, influenza C virus (ICV) is a ubiquitous childhood pathogen typically causing mild respiratory symptoms. ICV infections are rarely diagnosed and less research has been performed on it despite the virus being capable of causing severe disease in infants. Here we report on the isolation of a human ICV from a child with acute respiratory disease, provisionally designated C/Victoria/2/2012 (C/Vic). The full-length genome sequence and phylogenetic analysis revealed that the hemagglutinin-esterase-fusion (HEF) gene of C/Vic was derived from C/Sao Paulo lineage, while its PB2 and P3 genes evolved separately from all characterized historical ICV isolates. Furthermore, antigenic analysis using the hemagglutination inhibition (HI) assay found that 1947 C/Taylor virus (C/Taylor lineage) was antigenically more divergent from1966 C/Johannesburg (C/Aichi lineage) than from 2012 C/Vic. Structure modeling of the HEF protein identified two mutations in the 170-loop of the HEF protein around the receptor-binding pocket as a possible antigenic determinant responsible for the discrepant HI results. Taken together, results of our studies reveal novel insights into the genetic and antigenic evolution of ICV and provide a framework for further investigation of its molecular determinants of antigenic property and replication.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Medical Virology
Volume92
Issue number2
DOIs
StatePublished - Feb 1 2020

Bibliographical note

Funding Information:
We thank Megan Quast for outstanding technical help in virus growth study. Work done in the FL lab was supported in part by and NIH R01AI141889, SDSU AES 3AH-477, and SD 2010 Research Center (Biological Control and Analysis of Applied Photonics) Fund SJ163. Work performed in the RK lab was supported by Agriculture Experiment Station) Hatch grant number SD00H547-15.

Funding Information:
We thank Megan Quast for outstanding technical help in virus growth study. Work done in the FL lab was supported in part by and NIH R01AI141889, SDSU AES 3AH‐477, and SD 2010 Research Center (Biological Control and Analysis of Applied Photonics) Fund SJ163. Work performed in the RK lab was supported by Agriculture Experiment Station) Hatch grant number SD00H547‐15.

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

Keywords

  • antigenic evolution
  • genesis
  • influenza C virus
  • phylogenetic evolution

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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