Genetic basis of Neisseria gonorrhoeae lipooligosaccharide antigenic variation

R. J. Danaher, J. C. Levin, D. Arking, C. L. Burch, R. Sandlin, D. C. Stein

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


Neisseria gonorrhoeae lipooligosaccharide (LOS) undergoes antigenic variation at a high rate, and this variation can be monitored by changes in a strain's ability to bind LOS-specific monoclonal antibodies. We report here the cloning and identification of a gene, lsi-2, that can mediate this variation. The DNA sequence of lsi-2 has been determined for N. gonorrhoeae 1291, a strain that expresses a high-molecular-mass LOS, and a derivative of this strain, RS132L, that produces a truncated LOS. In the parental strain, lsi-2 contains a string of 12 guanines in the middle of its coding sequence. In cells that had antigenically varied to produce a truncated LOS, the number of guanines in lsi-2 was altered. Site-specific deletions were constructed to verify that expression of a 3.6-kDa LOS is due to alterations in lsi-2.

Original languageEnglish
Pages (from-to)7275-7279
Number of pages5
JournalJournal of Bacteriology
Issue number24
StatePublished - 1995

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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