TY - JOUR
T1 - Genetic manipulation of Streptomyces species
AU - Nybo, S. Eric
AU - Shepherd, Micah D.
AU - Bosserman, Mary A.
AU - Rohr, Jürgen
PY - 2010/11
Y1 - 2010/11
N2 - This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.
AB - This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.
KW - Conjugation
KW - Genomic DNA isolation
KW - Protoplast
KW - Streptomyces
KW - Transformation
UR - http://www.scopus.com/inward/record.url?scp=79952198612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952198612&partnerID=8YFLogxK
U2 - 10.1002/9780471729259.mc10e03s19
DO - 10.1002/9780471729259.mc10e03s19
M3 - Article
C2 - 21053253
AN - SCOPUS:79952198612
SN - 1934-8525
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
IS - SUPPL.19
M1 - 10E.3
ER -