Genetic manipulation of Streptomyces species

S. Eric Nybo, Micah D. Shepherd, Mary A. Bosserman, Jürgen Rohr

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.

Original languageEnglish
Article number10E.3
JournalCurrent Protocols in Microbiology
Issue numberSUPPL.19
StatePublished - Nov 2010


  • Conjugation
  • Genomic DNA isolation
  • Protoplast
  • Streptomyces
  • Transformation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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