Abstract
Rhizoctonia solani AG-2-2IIIB is an important seedling pathogen of soybean in North America and other soybean-growing regions around the world. There is no information regarding the population genetics of field populations of R. solani associated with soybean seedling disease. More specifically, information regarding genetic diversity, the mode of reproduction, and the evolutionary factors that shape different R. solani populations separated in time and space are lacking. We exploited genotyping by sequencing as a tool to assess the genetic structure of R. solani AG-2-2IIIB populations from Illinois, Ohio, and Ontario and investigate the reproductive mode of this subgroup. Our results revealed differences in genotypic diversity among three populations, with the Ontario population having greatest diversity. An overrepresentation of multilocus genotypes (MLGs) and a rejection of the null hypothesis of random mating in all three populations suggested clonality within each population. However, phylogenetic analysis revealed long terminal multifurcating branches for most members of the Ontario population, suggesting a mixed reproductive mode for this population. Analysis of molecular variance revealed low levels of population differentiation, and sharing of similar MLGs among populations highlights the role of genotype flow as an evolutionary force shaping population structure of this subgroup.
| Original language | English |
|---|---|
| Pages (from-to) | 2132-2141 |
| Number of pages | 10 |
| Journal | Phytopathology |
| Volume | 109 |
| Issue number | 12 |
| DOIs | |
| State | Published - 2019 |
Bibliographical note
Publisher Copyright:© 2019 The American Phytopathological Society
Funding
Funding: This work was supported by the United Soybean Board (to C. A. Bradley), the North Central Soybean Research Program (to C. A. Bradley), and Grain Farmers of Ontario. This work was supported by the United Soybean Board (to C. A. Bradley), the North Central Soybean Research Program (to C. A. Bradley), and Grain Farmers of Ontario. 1Department of Crop Sciences, University of Illinois, Urbana, IL 61801, U.S.A. 2Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, U.S.A. 3Ontario Ministry of Agriculture, Food and Rural Affairs, Ridgetown, Ontario N0P2C0, Canada 4Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, OH, U.S.A.
| Funders | Funder number |
|---|---|
| National Fisheries Research and Development Institute (NFRDI) | |
| Ohio State University | |
| University of Illinois | |
| United Soybean Board | |
| Grain Farmers of Ontario | |
| Ministry of Agriculture, Food and Rural Affairs |
Keywords
- Mycology, population biology
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science