TY - JOUR
T1 - Genetic Structure of Rhizoctonia solani AG-2-2IIIB from Soybean in Illinois, Ohio, and Ontario
AU - Ajayi-Oyetunde, Olutoyosi O.
AU - Everhart, Sydney E.
AU - Brown, Patrick J.
AU - Tenuta, Albert U.
AU - Dorrance, Anne E.
AU - Bradley, Carl A.
N1 - Publisher Copyright:
© 2019 The American Phytopathological Society
PY - 2019
Y1 - 2019
N2 - Rhizoctonia solani AG-2-2IIIB is an important seedling pathogen of soybean in North America and other soybean-growing regions around the world. There is no information regarding the population genetics of field populations of R. solani associated with soybean seedling disease. More specifically, information regarding genetic diversity, the mode of reproduction, and the evolutionary factors that shape different R. solani populations separated in time and space are lacking. We exploited genotyping by sequencing as a tool to assess the genetic structure of R. solani AG-2-2IIIB populations from Illinois, Ohio, and Ontario and investigate the reproductive mode of this subgroup. Our results revealed differences in genotypic diversity among three populations, with the Ontario population having greatest diversity. An overrepresentation of multilocus genotypes (MLGs) and a rejection of the null hypothesis of random mating in all three populations suggested clonality within each population. However, phylogenetic analysis revealed long terminal multifurcating branches for most members of the Ontario population, suggesting a mixed reproductive mode for this population. Analysis of molecular variance revealed low levels of population differentiation, and sharing of similar MLGs among populations highlights the role of genotype flow as an evolutionary force shaping population structure of this subgroup.
AB - Rhizoctonia solani AG-2-2IIIB is an important seedling pathogen of soybean in North America and other soybean-growing regions around the world. There is no information regarding the population genetics of field populations of R. solani associated with soybean seedling disease. More specifically, information regarding genetic diversity, the mode of reproduction, and the evolutionary factors that shape different R. solani populations separated in time and space are lacking. We exploited genotyping by sequencing as a tool to assess the genetic structure of R. solani AG-2-2IIIB populations from Illinois, Ohio, and Ontario and investigate the reproductive mode of this subgroup. Our results revealed differences in genotypic diversity among three populations, with the Ontario population having greatest diversity. An overrepresentation of multilocus genotypes (MLGs) and a rejection of the null hypothesis of random mating in all three populations suggested clonality within each population. However, phylogenetic analysis revealed long terminal multifurcating branches for most members of the Ontario population, suggesting a mixed reproductive mode for this population. Analysis of molecular variance revealed low levels of population differentiation, and sharing of similar MLGs among populations highlights the role of genotype flow as an evolutionary force shaping population structure of this subgroup.
KW - Mycology, population biology
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U2 - 10.1094/PHYTO-01-19-0015-R
DO - 10.1094/PHYTO-01-19-0015-R
M3 - Article
C2 - 31381483
AN - SCOPUS:85076123347
SN - 0031-949X
VL - 109
SP - 2132
EP - 2141
JO - Phytopathology
JF - Phytopathology
IS - 12
ER -