TY - JOUR
T1 - Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants
AU - Zhang, Yu
AU - Luo, Xiangwen
AU - Zhang, Deyong
AU - OuYang, Xian
AU - Zhang, Zhanhong
AU - Li, Fan
AU - Zhang, Chengjia
AU - Chen, Jianxin
AU - Zhou, Xuguo
AU - Zhang, Songbai
AU - Liu, Yong
N1 - Publisher Copyright:
© 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.).
PY - 2019/8/15
Y1 - 2019/8/15
N2 - In 2013, a chili pepper (Capsicum annuum cv. ‘Favorite’) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.
AB - In 2013, a chili pepper (Capsicum annuum cv. ‘Favorite’) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.
KW - Coat protein gene
KW - Complete genome sequence
KW - Pepper mottle virus
KW - Virus evolution
KW - Virus recombination
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U2 - 10.1007/s42161-018-00235-w
DO - 10.1007/s42161-018-00235-w
M3 - Article
AN - SCOPUS:85070854283
SN - 1125-4653
VL - 101
SP - 559
EP - 564
JO - Journal of Plant Pathology
JF - Journal of Plant Pathology
IS - 3
ER -