Genome editing of Wnt-1, a gene associated with segmentation, via CRISPR/Cas9 in the pine caterpillar moth, Dendrolimus punctatus

Huihui Liu, Qun Liu, Xuguo Zhou, Yongping Huang, Zhen Zhang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos and larvae showed an abnormal development. DpWnt-1 mutants predominantly exhibited abnormal posterior segments. In addition, multiple phenotypes were observed, including the loss of limbs and the head deformation, suggesting that DpWnt-1 signaling pathway is necessary for anterior segmentation and appendage development. Overall, our results demonstrate that CRISPR/Cas9 system is feasible and efficient in inducing mutations at a specific locus in D. punctatus. This study not only lays the foundation for characterizing gene functions in a non-model species, but also facilitates the future development of pest control alternatives for a major defoliator.

Original languageEnglish
Article number666
JournalFrontiers in Physiology
Volume7
Issue numberJAN
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017 Liu, Liu, Zhou, Huang and Zhang.

Keywords

  • CRISPR/Cas9
  • Dendrolimus punctatus
  • Embryogenesis
  • Genome editing
  • Segmentation
  • Wnt-1

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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